An oxidative damage in cell structures is a basis of most mechanisms that lead to health diseases and senescence of human body. The presence of antioxidant issues such as redox potential imbalance in human body is a very important question for modern clinical diagnostics. Implementation of multispectral and colour analysis of the human skin into optical diagnostics of such wide distributed in a human body antioxidant as ubiquinone can be one of the steps for development of the device with a view to clinical diagnostics of redox potential or quality control of the cosmetics. The recording of multispectral images of the hand skin with monochromatic camera and a set of coloured filters was provided in the current research. Recording data of the multispectral imaging technique was processed using principal component analysis. Also colour characteristics of the skin before and after the skin treatment with facial mask which contains ubiquinone were calculated. The results of the mask treatment were compared with the treatment using oily ubiquinone solution. Despite the fact that results did not give clear explanation about healthy skin or skin stressed by reactive oxygen species, methods which were described in this research are able to identify how skin surface is changing after the antioxidant treatment. In future it is important to provide biomedical tests during the optical tests of the human skin.
The presence of antioxidant issues such as redox potential imbalance in human body is a very important question for modern clinical diagnostics. Implementation of fluorescence analysis into optical diagnostics of such wide distributed in a human body antioxidant as ubiquinone is one of the steps for development of the device with a view to clinical diagnostics of redox potential. Recording of fluorescence was carried out with spectrometer using UV irradiation source with thin band (max at 287 and 330 nm) as a background radiation. Concentrations of ubiquinone from 0.25 to 2.5 mmol/l in explored samples were used for investigation. Recording data was processed using correlation analysis and differential analytical technique. The fourth derivative spectrum of fluorescence spectrum provided the basis for a multicomponent analysis of the solutions. As a technique in clinical diagnostics fluorescence analysis with processing method including differential spectrophotometry, it is step forward towards redox potential calculation and quality control in pharmacy for better health care.
Antioxidants, reactive oxygen species and other radicals are involved in oxidative stress phenomena. There are many available methods to quantify the progress of the process and evidence its existence for each stage of oxidative damage process which can be classified into direct and indirect approaches. The technic of experimental determination of optical characteristics stability of antioxidant solutions with spectroscopic method was offered in this work as a direct method for investigation of color stability in time and fluorescence behavior of antioxidant solutions. Coenzyme Q10, or ubiquinone, in the form of oil and alcohol solutions was chosen as an antioxidant of research. The experimental results connected with the optical density and color characteristics confirm the possibility of applying the clinical diagnosis method of estimation redox potential. Also, future prospects, including application of this technic in pharmaceutical analysis for quality control, were discussed.
This work is dedicated to the research of the clinical diagnosis method of determining the antioxidant status of the human body. The existing methods for determining the level of antioxidants in connection with biological functions of the human antioxidant system were studied. Antioxidants in the human body, in the form of solutions were chosen as a research objects. The technique of experimental studies of ubiquinone solutions in oil and alcohol by spectroscopic method was offered. The experimental results connected with the optical density and color characteristics confirm the possibility of applying the clinical diagnosis method of estimation antioxidant balance. Also, it was found that this method can be applicable to the quality control of medicines for treating diseases provoked by oxidative stress, which means that this method may be developed not only for using in the clinic.
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