Active fluorescence detection is a common detection method. A fluorescence-lifetime parameter is used as the characteristic parameter of active fluorescence detection, which can solve that the fluorescence intensity is easily affected by external environmental factors. Analyzed the principle of the gated-detection method for measuring the fluorescence life, the nonlinear least square regress combined with fluorescence-lifetime decay curve was proposed to fit the function of fluorescence-lifetime decay. Extracting the average fluorescence-lifetime parameters, the fluorescence life map was drawn for the two-dimensional spatial distribution of fluorescence substances. An identification method of oil types was presented and verified by experiment using fluorescence-lifetime parameter as feature vector and support vector machine. The experimental results shown that the probability of the pixel point fluorescence-lifetime falling into the confidence interval in the excitation region was over 68% by using the fluorescence average life as a parameter, and the identification probability was over 77% by using the support vector machine for oil species identification. It is feasible and has a good identification rate to identify oil species by using fluorescence-lifetime parameter. Few samples are trained by combining the identification method of support vector machine. The method based on fluorescence-lifetime decay curve combined with support vector machine will provide some references for oil species recognition research in the field of environmental pollution.
Active Induced fluorescence technique is a widely used non-destructive detection method. An economical and efficient chlorophyll fluorescence excitation system was designed by analyzing the number of LEDs and the spatial irradiance distribution in different directions. Irradiance distribution of different LED quantities was simulated, and the irradiance uniformity of the sample area was analyzed under different light sources and different distances. The ratio of the minimum irradiance to the average irradiance in the sample area was proposed as an indicator of the irradiance uniformity. When the number of LEDs was 4, the spacing was 0.04m, and the LED array height was 0.5m, the plane irradiance uniformity was about 0.920 according simulation analysis. The simulation results show that the uniformity of irradiance was positively correlated with the LED array height and LED spacing. The irradiance uniformity increases with the increase of LED number. It can be used as a reference for the design of different LED arrays, and provide ideas for the design of active fluorescence excitation system in the future.
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