KEYWORDS: Light sources and illumination, Microscopes, Digital micromirror devices, Data processing, Control systems, Brain, Telecommunications, Real-time computing, Optogenetics, Neuroscience
We introduce the Spatiotemporal Illumination Microscope (STIMscope) designed for cellular-resolution, large field-of-view imaging and patterned illumination for neuroscience applications. Integrated with a centralized control and processing unit, the STIMscope features a closed-loop real-time analysis pipeline tailored for neural dynamic imaging and manipulation. We demonstrate its versatility by creating application-dependent versions of the platform and evaluating their performance. Additionally, we highlight its efficacy in large field-of-view neural imaging and targeted optogenetics of cortical organotypic cultures.
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