The retinal vasculature has plexuses at multiple depths, interconnected in a complex pattern. While optical coherence tomography angiography (OCTA) visualizes the vasculature at the capillary level, creating a 3D representation of the vasculature, including the connections between plexuses, remains a challenge. Here, we employ Optimally Oriented Flux (OOF) on retinal OCTA to simultaneously preserve the inter-plexus connections and suppress projection artifacts, facilitating high-contrast 3D visualization of the vasculature. Furthermore, a novel framework is developed to transform the vasculature into a graph, which enables quantification of ‘importance’ of each capillary using betweenness centrality and simulation of capillary non-perfusion.
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