Dr. Hongwu Tang Profile

Dr. Hongwu Tang

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Proceedings Article | 29 October 2009 Paper

Probing of marker proteins in cancer tissue using quantum dots with Hadamard transform fluorescence imaging microscopy

Hao Xu, Chuang Chen, Yan Li, Hong-Wu Tang

Proceedings Volume 7519, 751917 (2009) https://doi.org/10.1117/12.841819

KEYWORDS: Luminescence, Tissues, Proteins, Breast cancer, Tumors, Imaging spectroscopy, Quantum dots, Microscopes, Cancer, Imaging systems

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A domestic-made Hadamard transform spectral imaging microscope was employed to provide high-resolutional fluorescence spectrum and image of tiny samples such as single cells and tissues. By using agron laser line at 454 nm to excite fluorescence and based on immunostaining with quantum dots (QDs) at different wavelengths to tag and trace breast cancer biomarkers in human breast cancer tissues, in situ single-color and dual-color fluorescence imaging for human epidermal growth factor receptor 2 (HER2), estrogen receptor (ER) and proliferating cell nuclear antigen (PCNA) in tissues were realized, by using the Hadamard imaging microscope to capture the high S/N ratio fluorescence images. Moreover, through the comparative study of the differences between fluorescence spectra and images of positive samples and negative control, a method was proposed to evaluate tumor malignancy of the specimens based on the analysis of distribution of HER2, ER and PCNA in the tissues. The results show that the Hadamard transform spectral imaging technique can be applied to visualize and quantitatively measure the subcellular molecules inside the tumor tissues and has great potential in biology and medical diagnosis.

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