Stabilizing the electric field in few cycle ultrashort laser pulses is necessary for performing many experiments in the attosecond time regime and for generating High-Harmonics (HHG). Traditional CEP methods are based on measuring the carrier envelope offset frequency and using an external feedback mechanism (pump power, intracavity dispersion, etc.) to stabilize the phase in femtosecond oscillators and amplifiers. In this talk, a novel feed-forward method for stabilizing a <25 fs, mJ-level regenerative amplifier is presented. Using this patented technique, <100 mrad (10-shot average) and <300 mrad single-shot noise performance was demonstrated for more than 150 hours. Details of this novel feed-forward approach will be discussed, and the results compared to other currently available methods.
Multiphoton microscopy has seen widespread adoption from the research community to address the challenges associated with image collection many hundreds of micrometers into living tissue. Over time, technology advancements have contributed to the evolution of 2-photon, 3-photon and other nonlinear microscopy techniques. Deep Tissue imaging necessitates wavelengths spanning from the visible to the near-IR to achieve desirable resolution. Excitation requires a femtosecond laser pulse to minimize out-of-focus fluorescence. Image collection requires novel broadband microscope objectives which must transmit both the near-IR excitation wavelengths and the subsequent visible fluorescence. During this presentation, a concise overview of the historical development of these technologies will be presented through real-life examples that demonstrate how modern research facilities are leveraging these advancements.
Hands-free ultrafast lasers have become the primary light source for many biological imaging applications. Multiphoton imaging has benefitted from these developments by offering an easy-to-use platform that reaches a wider audience. Techniques such as Fluorescent Resonant Energy Transfer (FRET), Fluorescence Lifetime Imaging Microscopy (FLIM), Coherent Anti-Stokes Raman Scattering (CARS), and Stimulated Raman Scattering (SRS) can easily be performed using these powerful tools. In this presentation, the latest developments in ultrafast lasers for multiphoton imaging will be presented.
Ultrafast lasers have enabled a number of advanced imaging modalities, including two-photon fluorescence excitation (TPFE), Coherent Anti-Stokes Raman Scattering (CARS), Stimulated Raman Scattering (SRS), Second Harmonic Generation (SHG) imaging, and Fluorescence Lifetime Imaging Microscopy (FLIM). The core principle that governs this important body of research is multiphoton absorption of a femtosecond laser pulse that allows for 2 or more photons being simultaneously absorbed. Images are produced from the resulting fluorescence. Over the years, multiphoton light sources have become more powerful, more tunable, and more accessible to more users. We will present the latest light source technologies with special focus on the experiments and images enabled by these technologies including neuroscience, cell biology, and optogenetics.
In this talk we first review the historical development of commercial mode-locked lasers based on titanium doped sapphire, including experimental and theoretical data highlighting the limits of these lasers, especially average output power and tuning range. Commercially available one-box systems are rapidly approaching these limits, with the latest systems offering an average power of more than 2.9W at 800nm, corresponding to more than 350kW peak power. In addition, systems are now becoming available with an extremely wide tuning range, extending from just under 700nm to over 1020nm, using only a single set of optics. These achievements enable further advancements of applications, such as micromachining, which require the highest peak power with increased throughput rates, and multi-photon microscopy where increased tunability and higher average power are of particular benefit. Some of the remaining challenges and the innovative techniques used to address them will also be discussed during the presentation.
In this talk we will present an overview of recent development of ultrafast lasers sources and their applications. This talk
will highlight some recent state of the art ultrafast pulse results from Ti:Sapphire and Ytterbium based laser systems.
There are significant advantages in being able to directly diode pump Ytterbium materials resulting in more compact
bulk solid state and fiber based laser systems. Several newly emerging technologies such as Optical Parametric Chirped
Pulse Amplification, and Supercontinuum Generation have generated great excitement in recent years. The evolution of
more compact and user friendly ultrafast laser systems has enabled completely new fields that take advantage of the
extremely high peak powers and very short time duration of ultrafast laser pulses. Recent results in the fields of
multiphoton microscopy, micromachining, 3-D fabrication, and spectroscopy will be discussed.
We report on the latest advances at Spectra-Physics in tunability and average power for automated and manual Ti:Sapphire laser sources that can be used for multiphoton microscopy - the Mai Tai® HP and Tsunami® HP. We also present new performance data for a fully automated Optical Parametric Oscillator - the Opal® pumped using the automated Ti:Sapphire pump source - Mai Tai HP.
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