Dr. James B. Delehanty Profile

Dr. James B. Delehanty

Researcher at US Naval Research Lab

SPIE Involvement:

Conference Program Committee | Author

Publications (25)

Proceedings Article | 7 March 2019 Presentation + Paper

Controlled membrane depolarization through photothermal effects of tethered gold nanoparticles

Megan Muroski, Okhil Nag, Eunkeu Oh, Alan Huston, James Delehanty

Proceedings Volume 10892, 108920M (2019) https://doi.org/10.1117/12.2508662

KEYWORDS: Nanoparticles, Gold, Plasmonics, Photothermal effect, Tumors, Nanomaterials, Live cell imaging, Statistical analysis, Action potentials, Neurons

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Proceedings Article | 23 February 2018 Presentation + Paper

Liquid crystal nanoparticles for delivery of photosensitizers for photodynamic therapy

Okhil Nag, Jawad Naciri, James Delehanty

Proceedings Volume 10507, 1050711 (2018) https://doi.org/10.1117/12.2289970

KEYWORDS: Picosecond phenomena, Photodynamic therapy, Nanoparticles, Liquid crystals, Plasma, Oxygen, Particles, Fluorescence resonance energy transfer, Toxicity, Tissues

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Proceedings Article | 23 February 2018 Presentation + Paper

Nanoparticle bioconjugate for controlled cellular delivery of doxorubicin

Ajmeeta Sangtani, Eleonora Petryayeva, Miao Wu, Kimihiro Susumu, Eunkeu Oh, Alan Huston, Guillermo Lasarte-Aragones, Igor Medintz, W. Russ Algar, James Delehanty

Proceedings Volume 10507, 105070M (2018) https://doi.org/10.1117/12.2290031

KEYWORDS: Toxicity, Nanoparticles, Modulation, Chemistry, Control systems, Quantum dots

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Proceedings Article | 22 February 2017 Presentation + Paper

Semiconductor quantum dots as Förster resonance energy transfer donors for intracellularly-based biosensors

Lauren Field, Scott Walper, Kimihiro Susumu, Eunkeu Oh, Igor Medintz, James Delehanty

Proceedings Volume 10078, 100780N (2017) https://doi.org/10.1117/12.2250899

KEYWORDS: Sensors, Quantum dots, Fluorescence resonance energy transfer, Semiconductors, Resonance energy transfer, Nanostructures, Fluorescent proteins, Biosensors, Live cell imaging, Proteins, Molecules, Optical properties

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Proceedings Article | 22 April 2016 Presentation + Paper

Membrane-targeting liquid crystal nanoparticles (LCNPs) for drug delivery

Okhil Nag, Jawad Naciri, Christopher Spillmann, James Delehanty

Proceedings Volume 9722, 972215 (2016) https://doi.org/10.1117/12.2212529

KEYWORDS: Liquid crystals, Nanoparticles, Plasma, Cell mechanics, Imaging systems, Modulation, Physiology, Particles, Neptunium, Polymers

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Proceedings Article | 22 April 2016 Paper

Imaging cellular membrane potential through ionization of quantum dots

Clare Rowland, Kimihiro Susumu, Michael Stewart, Eunkeu Oh, Antti Mäkinen, Thomas O'Shaughnessy, Gary Kushto, Mason Wolak, Jeffrey Erickson, Alexander Efros, Alan Huston, James Delehanty

Proceedings Volume 9722, 97220S (2016) https://doi.org/10.1117/12.2207337

KEYWORDS: Quantum dots, Ionization, Action potentials, Absorption, Luminescence, Neurons, Brain, Electrodes, Silica, Capacitors

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Proceedings Article | 22 April 2016 Presentation + Paper

The influence of cell penetrating peptide branching on cellular uptake of QDs

Joyce Breger, James Delehanty, Kimihiro Susumu, George Anderson, Markus Muttenhaler, Philip Dawson, Igor Medintz

Proceedings Volume 9722, 97220R (2016) https://doi.org/10.1117/12.2207311

KEYWORDS: Nanoparticles, Quantum dots, Semiconductors, Dendrimers, Sensors, Neptunium, Carbon dioxide, Analytical research, Luminescence, Toxicity, Tissues, Biosensing

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Proceedings Article | 21 August 2015 Paper

Semiconductor quantum dots as delivery and imaging platforms for intracellular assembly

Lauren Field, James Delehanty, Scott Walper, Kimihiro Susumu, Igor Medintz

Proceedings Volume 9550, 955002 (2015) https://doi.org/10.1117/12.2188392

KEYWORDS: Fluorescence resonance energy transfer, Quantum dots, Proteins, Semiconductors, Nanolithography, Molecules, Neodymium, Visualization, Optical properties, Ions

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Proceedings Article | 12 March 2015 Paper

Membrane-targeting peptides for nanoparticle-facilitated cellular imaging and analysis

Joyce Breger, James Delehanty, Kelly Boeneman Gemmill, Lauren Field, Juan Blanco-Canosa, Philip Dawson, Alan Huston, Igor Medintz

Proceedings Volume 9338, 93381P (2015) https://doi.org/10.1117/12.2077026

KEYWORDS: Plasma, Nanoparticles, Luminescence, Analytical research, Quantum dots, Nanomaterials, Metals, Zinc, Biological research, Physiology

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Proceedings Article | 10 March 2015 Paper

Visualization and neuronal cell targeting during electrophysiological recordings facilitated by quantum dots

Lauren Field, Bertalan Andrásfalvy, Gregorio Galiñanes, Daniel Huber, Mladen Barbic, John Macklin, Kimihiro Susumu, James Delehanty, Alan Huston, Judit Makara, Igor Medintz

Proceedings Volume 9305, 93050Y (2015) https://doi.org/10.1117/12.2076934

KEYWORDS: Neurons, Visualization, Quantum dots, Plasma, Tissues, Brain, In vivo imaging, Coating, Thin film coatings, Physiology

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Proceedings Article | 27 August 2014 Paper

Controlling the intracellular fate of nano-bioconjugates: pathways for realizing nanoparticle-mediated theranostics

James Delehanty, Juan Blanco-Canosa, Christopher Bradburne, Kimihiro Susumu, Michael Stewart, Duane Prasuhn, Philip Dawson, Igor Medintz

Proceedings Volume 9166, 91660A (2014) https://doi.org/10.1117/12.2064372

KEYWORDS: Neptunium, Plasma, Nanomaterials, Proteins, Quantum dots, Gold, Nanoparticles, Imaging systems, Toxicity, Analytical research

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Proceedings Article | 29 May 2013 Paper

Peptide-mediated cellular delivery of semiconductor quantum dots

Kelly Boeneman Gemmill, Markus Muttenthaler, James Delehanty, Jeff Deschamps, Kimihiro Susumu, Michael Stewart, Philip Dawson, Alan Huston, Igor Medintz

Proceedings Volume 8723, 872311 (2013) https://doi.org/10.1117/12.2015446

KEYWORDS: Quantum dots, Nanoparticles, Biosensing, Semiconductors, Proteins, Image analysis, Mirrors, Toxicity, Fluorescent proteins, In vitro testing

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Proceedings Article | 22 February 2013 Paper

Recent development of dihydrolipoic acid appended ligands for robust and biocompatible quantum dots

Kimihiro Susumu, Eunkeu Oh, Michael Stewart, James Delehanty, Kelly Gemmill, Vaibhav Jain, Alan Huston, Igor Medintz

Proceedings Volume 8595, 859515 (2013) https://doi.org/10.1117/12.2004952

KEYWORDS: Luminescence, Quantum dots, Proteins, Nanoparticles, Quantum efficiency, Zinc, Semiconductors, Coating, Adsorption, Biosensing

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Proceedings Article | 22 February 2013 Paper

Fluorescent nanocolloids for differential labeling of the endocytic pathway and drug delivery applications

James Delehanty, Christopher Spillmann, Jawad Naciri, W. Russ Algar, Banahalli Ratna, Igor Medintz

Proceedings Volume 8595, 85951E (2013) https://doi.org/10.1117/12.2007749

KEYWORDS: Proteins, Tissues, Nanoparticles, Iron, Biomedical optics, Quantum dots, Target recognition, Liquid crystals, Control systems, Nanomaterials

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Proceedings Article | 2 February 2012 Paper

Further progress in cytosolic cellular delivery of quantum dots

Kelly Boeneman Gemmill, James Delehanty, Michael Stewart, Kimihiro Susumu, Juan Blanco-Canosa, Philip Dawson, Alan Huston, Igor Medintz

Proceedings Volume 8232, 82320B (2012) https://doi.org/10.1117/12.906801

KEYWORDS: Quantum dots, Nanoparticles, Proteins, Biosensing, Toxicity, Luminescence, Polymers, Manufacturing, Zinc, In vivo imaging

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Proceedings Article | 5 May 2010 Paper

Quantum dots in life sciences: applications, benefits, and safety issues

James Delehanty, Christopher Bradburne, Kelly Boeneman, Kimihiro Susumu, Bing Mei, Juan Blanco-Canosa, Phillip Dawson, Hedi Mattoussi, Alan Huston, Igor Medintz

Proceedings Volume 7666, 766619 (2010) https://doi.org/10.1117/12.853121

KEYWORDS: Quantum dots, Life sciences, Safety, Semiconductors, Spectroscopy, Quantum efficiency, Polymers, Analytical research, Biology, Optical spectroscopy

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Proceedings Article | 18 February 2010 Paper

Delivery of quantum dot bioconjugates to the cellular cytosol: release from the endolysosomal system

James Delehanty, Christopher Bradburne, Kelly Boeneman, Igor Medintz, Dorothy Farrell, Thomas Pons, Bing Mei, Juan Blanco-Canosa, Philip Dawson, Hedi Mattoussi

Proceedings Volume 7575, 75750S (2010) https://doi.org/10.1117/12.842434

KEYWORDS: Polymers, Quantum dots, Proteins, Semiconductors, Plasma, Analytical research, Toxicity, Tissues, Sodium, Cell biology

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To realize their full potential as intracellular imaging and sensing reagents, robust and efficient methods for the targeted cellular delivery of luminescent semiconductor quantum dots (QDs) must be developed. We have previously shown that QDs decorated with histidine-terminated polyarginine cell-penetrating peptides (CPP) are rapidly and specifically internalized via endocytosis by several mammalian cell lines with no cytotoxicity. Here we demonstrate the long-term intracellular stability and fate of these QD-peptide conjugates. We found that the QD-peptide conjugates remain sequestered within endolysosomal vesicles for up to three days after delivery. However, the CPP appeared to remain stably associated with the QD within these acidic vesicles over this time period. Hence, we explored a number of techniques to either actively deliver QDs directly to the cytosol or to facilitate the endosomal release of endocytosed QDs to the cytosol. Active methods (e.g., electroporation) delivered only modest amounts of QDs to the cytosol that appeared to form aggregates. Delivery of QDs using polymer-based transfection reagents resulted primarily in the endosomal sequestration of the QDs, although one commercial polymer tested delivered QDs to the cytosol but only after several days in culture and with a considerable degree of polymer-induced toxicity. Finally, a modular, amphiphilic peptide containing functionalities designed for cell penetration and vesicular membrane interaction demonstrated the ability to deliver QDs in a well-dispersed manner to the cytosol. This peptide mediated rapid QD uptake followed by a slower efficient endosomal release of the QDs to the cytosol that peaked at 48 hours post-delivery. Importantly, this QD-peptide conjugate elicited minimal cytotoxicity in two cell lines tested. A more detailed understanding of the mechanism of the peptide's uptake and endosomal escape attributes will lead to the design of further QD conjugates for targeted imaging and sensing applications.

Proceedings Article | 3 March 2009 Paper

Intracellular delivery of and sensing with quantum dot bioconjugates

James Delehanty, Christopher Bradburne, Igor Medintz, Dorothy Farrell, Thomas Pons, Jeffrey Deschamps, Florence Brunel, Philip Dawson, Hedi Mattoussi

Proceedings Volume 7189, 71890W (2009) https://doi.org/10.1117/12.809286

KEYWORDS: Polymers, Quantum dots, Toxicity, Luminescence, Analytical research, Semiconductors, Quantum efficiency, Absorption, Multiplexing, Receptors

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Proceedings Article | 3 March 2009 Paper

Peptide linkers for the assembly of semiconductor quantum dot bioconjugates

Kelly Boeneman, Bing Mei, Jeffrey Deschamps, James Delehanty, Hedi Mattoussi, Igor Medintz

Proceedings Volume 7189, 71890G (2009) https://doi.org/10.1117/12.808540

KEYWORDS: Proteins, Fluorescence resonance energy transfer, Quantum dots, Semiconductors, In vitro testing, Gold, Nanolithography, Quantum efficiency, Distance measurement, Fluorescent proteins

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Proceedings Article | 13 March 2008 Paper

Assembly and intracellular delivery of quantum dot-fluorescent protein bioconjugates

Igor Medintz, Thomas Pons, James Delehanty, Kimihiro Susumu, Philip Dawson, Hedi Mattoussi

Proceedings Volume 6866, 68660B (2008) https://doi.org/10.1117/12.763702

KEYWORDS: Proteins, Fluorescence resonance energy transfer, Luminescence, Quantum dots, Sensors, Nanocrystals, Fluorescent proteins, Nanolithography, Nanoparticles, Digital image correlation

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Proceedings Article | 26 February 2008 Paper

Specific cellular delivery and intracellular fate of quantum dot-peptide and quantum dot-polymer nanoassemblies

James Delehanty, Christopher Bradburne, Igor Medintz, Dorothy Farrell, Thomas Pons, Florence Brunel, Philip Dawson, Hedi Mattoussi

Proceedings Volume 6866, 68660K (2008) https://doi.org/10.1117/12.763760

KEYWORDS: Polymers, Quantum dots, Luminescence, Toxicity, Ions, Biology, Semiconductors, Biomedical optics, Spectroscopy, Metals

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Proceedings Article | 22 February 2008 Paper

New ligand design to promote water compatibility of luminescent quantum dots and gold nanoparticles

Bing Mei, Kimihiro Susumu, Igor Medintz, James Delehanty, Hedi Mattoussi

Proceedings Volume 6866, 686606 (2008) https://doi.org/10.1117/12.764267

KEYWORDS: Nanoparticles, Gold, Quantum dots, Nanocrystals, Absorption, Luminescence, Nitrogen, Sodium, Optical properties, Silica

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Proceedings Article | 27 February 2007 Paper

Selective cellular delivery of self-assembled quantum dot-peptide bioconjugates

James Delehanty, Igor Medintz, Thomas Pons, Philip Dawson, Florence Brunel, Hedi Mattoussi

Proceedings Volume 6448, 64480U (2007) https://doi.org/10.1117/12.700768

KEYWORDS: Luminescence, Toxicity, Quantum dots, Control systems, Quantum efficiency, Semiconductors, Absorption, Sodium, Signal detection, Analytical research

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Proceedings Article | 22 July 2003 Paper

An automated portable array biosensor

Joel Golden, Yura Shubin, Lisa Shriver-Lake, James Delehanty, Kim Sapsford, Chris Rowe-Taitt, Frances Ligler

Proceedings Volume 4958, (2003) https://doi.org/10.1117/12.485497

KEYWORDS: Biosensors, Waveguides, Sensors, Molecules, Biological research, Receptors, Pathogens, Statistical analysis, Safety, Defense and security

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Proceedings Article | 22 February 2002 Paper

Array biosensor for environmental monitoring

Frances Ligler, K. Sapsford, Y. Shubin, J. Lemmond, Chris Rowe-Taitt, J. Delahanty, Joel Golden

Proceedings Volume 4576, (2002) https://doi.org/10.1117/12.456949

KEYWORDS: Biosensors, Waveguides, Statistical analysis, Luminescence, Biological research, Sensors, Environmental monitoring, CCD image sensors, Planar waveguides, Analytical research

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Showing 5 of 25 publications

Conference Committee Involvement (17)

Colloidal Nanoparticles for Biomedical Applications XX

25 January 2025 | San Francisco, California, United States

Colloidal Nanoparticles for Biomedical Applications XIX

27 January 2024 | San Francisco, California, United States

Colloidal Nanoparticles for Biomedical Applications XVIII

28 January 2023 | San Francisco, California, United States

Colloidal Nanoparticles for Biomedical Applications XVII

22 January 2022 | San Francisco, California, United States

Colloidal Nanoparticles for Biomedical Applications XVI

6 March 2021 | Online Only, California, United States

Colloidal Nanoparticles for Biomedical Applications XV

1 February 2020 | San Francisco, California, United States

Colloidal Nanoparticles for Biomedical Applications XIV

2 February 2019 | San Francisco, California, United States

Colloidal Nanoparticles for Biomedical Applications XIII

27 January 2018 | San Francisco, California, United States

Colloidal Nanoparticles for Biomedical Applications XII

28 January 2017 | San Francisco, California, United States

Colloidal Nanoparticles for Biomedical Applications XI

13 February 2016 | San Francisco, California, United States

Advances in Global Health through Sensing Technologies 2015

20 April 2015 | Baltimore, MD, United States

Colloidal Nanoparticles for Biomedical Applications X

7 February 2015 | San Francisco, California, United States

Sensing Technologies for Global Health, Military Medicine, and Environmental Monitoring IV

5 May 2014 | Baltimore, MD, United States

Colloidal Nanoparticles for Biomedical Applications IX

1 February 2014 | San Francisco, California, United States

Sensing Technologies for Global Health, Military Medicine, and Environmental Monitoring III

29 April 2013 | Baltimore, Maryland, United States

Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring II

23 April 2012 | Baltimore, Maryland, United States

Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring

25 April 2011 | Orlando, Florida, United States

Showing 5 of 17 Conference Committees

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