Margin status directly correlates with patient survival in many types of cancer. To improve the identification of positive margins, we report a rapid surgical guidance workflow including tissue staining, open-top light-sheet (OTLS) microscopy imaging, and post-processing to generate en face histologic images of fresh tissue surfaces. Compared with conventional frozen section analysis (FSA) in which a few vertical sections of tissue are sampled, our technology could comprehensively image large margin surfaces in a non-destructive manner and achieve superior image quality. We provide examples showing that the image quality generated by our rapid surface-histology method approaches that of gold-standard archival histology.
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