In-vivo imaging of the retina inevitably requires an image acquisition through the subject's pupil, which suffers from aberrations induced by the subject's crystalline lens and cornea, as well as scattering by tissue. Epi-illumination differential phase contrast (DPC) has demonstrated recovery phase information of cellular structures from intensity images captured with oblique back-illumination; but its image quality can still be compromised by eye movements and aberration. To overcome these challenges, we propose a computational method that compensates for the aberration in the platform of Oblique back-illumination differential phase-contrast microscopy. Our method involves asymmetric oblique-back illumination, so that phase images can be obtained with smaller number of acquisitions. The acquired images are then processed with a novel gradient decent-based algorithm to obtain aberration-corrected images and pupil aberration simultaneously. We will present the details of our experimental setup and reconstruction algorithms.
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