Ms. Jingtao Li Profile

Jingtao Li

at Harbin Institute of Technology

SPIE Involvement:

Author

Publications (2)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 7 March 2019 Paper

Super-resolution scanning microscopy with virtually structured illumination

Su Zhang, Jingtao Li, Limin Zou, Xuemei Ding

Proceedings Volume 11053, 110532C (2019) https://doi.org/10.1117/12.2511907

KEYWORDS: Microscopy, Modulation, Super resolution, Point spread functions, Spatial frequencies, Optical transfer functions, Diffraction, Microscopes, Multiphoton microscopy, Image processing

Read Abstract +

The resolution of optical microscopy fundamentally limited by diffraction is at best 200 nm. Super-resolution structured illumination microscopy (SR-SIM) provides an elegant way of overcoming the diffraction limit in conventional widefield microscope by superimposing a grid pattern generated through interference of diffraction orders on the specimen while capturing images. The use of non-uniform illumination field “shift” high specimen frequencies which are out-ofband into the pass-band of the microscope through spatial frequency mixing with the illumination field. Therefore the effective bandwidth of SR-SIM is approximately twice as conventional microscopy, corresponding to a 2-fold resolution enhancement, if the difference between excitation and emission wavelength is ignored. However, such a wide-field scheme typically can only image optically thin samples and is incompatible with multiphoton processes. In this paper, we propose a Super-resolution scanning scheme with virtually structured illumination, utilizes detection sensitivity modulation on line by programming or off line by numerical processing together with temporally cumulative imaging, the excitation intensity is constant while capturing images. In this case a nondescanned array detector such as CCD camera is needed. When combined with multiphoton excitation, this scheme can image thick samples with threedimensional optical sectioning and much improved resolution.

Proceedings Article | 7 March 2019 Paper

Research on key technologies of quadrupole electromagnetic tweezer

He Ni, Jingtao Li, Limin Zou, Peng Zhang, Xuemei Ding

Proceedings Volume 11053, 110532I (2019) https://doi.org/10.1117/12.2511935

KEYWORDS: Magnetism, Control systems, Electromagnetism, Feedback control, Liquids, Microscopes, Motion models, Imaging systems, Superposition, Luminescence

Read Abstract +

In this paper, the quadrupole electromagnetic tweezer installed in a fluorescent microscope was developed for the purpose of achieving precise control of magnetic microspheres’ motion trajectory. The key technologies of magnetic microsphere control and positioning by such quadrupole magnetic tweezers were systematically studied. An electromagnetic quadrupole magnetic tweezer system was designed and constructed, a current-magnetic force model of the quadrupole magnetic tweezer was established, and the magnetic force-current inverse force model was derived and simplified. A fluorescence microscopy imaging system was set up and the related program design was completed. The position of magnetic fluorescent microspheres was monitored by a high-speed CCD with sampling frequency of 200 Hz. A proportional-integral closedloop feedback controller was built up for magnetic microspheres. The experimental results demonstrated that the magnetic force range available at the center of the work area was [-80pN, 80pN]. Besides, magnetic microspheres were tested to possess a displacement resolution up to 400 nm as well as the capacity of moving in any direction in a two-dimensional plane. Based on the obtained results, it is expected that the quadrupole electromagnetic tweezer can function one of the effective devices for evaluation of cell mechanical properties.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years