KEYWORDS: Wavefronts, Thermal optics, Fluorescence microscopy, Resistors, Fluorescence imaging, Fluorescence, 3D image processing, Zernike polynomials, Thermography, Signal to noise ratio
We recently proposed a new wavefront shaping concept, coined as Smartlens, in which the phase of the transmitted light is shaped by engineering the temperature landscape in a thermo-optical material. Individually or in arrays, these microscale devices can generate complex functions based on either pure, or a combination of, Zernike polynomials, including lenses or electrically-tuneable aberration correctors. We will see how this concept could complement the existing optical shaping toolbox by offering low-chromatic-aberration, polarization-insensitive and transmission-mode micro-components. I will then delve into the potential of a single reconfigurable Smartlens to embed adaptive optics within a fluorescence endoscope. Finally, I will show that a Smartlens Array can enable multiplane Ca2+ or voltage imaging in Zebrafish at KHz frame rates.
We present a novel plasmonic sensor configuration that allows the discrimination of chiral molecules. The sensor consists of handed gold nanostructures of gammadion shape, distributed in a racemic (50/50 mixture) matrix with C4 symmetry. Its optical response enhances the interaction with molecules thus circular dichroism can be measured in the visible range. The bare sensors exhibit a flat CD signal, providing background-free CD measurements for molecular detection. We have used a chiral molecular model based on L-, D-, and the racemic mixture of phenylalanine, which allows us to evaluate the opposite chiral effects while having a reference system. Additionally, we have used molecular thermal evaporation technique to deposit a dense molecular layer on top of the sensors in a controllable and reproducible way. Our results show the discrimination of phenylalanine enantiomers through positive or negative peaks while the racemic mixture shows a flat signal. In addition, we present preliminary results that show that this approach is also suitable for microfluidics systems with a much lower density of chiral molecules.
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