Dr. Kazuhiro Gesho Profile

Dr. Kazuhiro Gesho

at Kagawa Univ

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Proceedings Article | 29 August 2008 Paper

Study on the floating micro-probe with multi degrees of freedom and function for cell operation: juggling probe

Kazuhiro Gesho, Tomohiro Uraki, Ichirou Ishimaru

Proceedings Volume 7038, 703812 (2008) https://doi.org/10.1117/12.794440

KEYWORDS: Microfluidics, Surgery, Fluid dynamics, Optical tweezers, 3D image processing, Resistance, Spectroscopy, Yeast, Mirrors, 3D metrology

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We develop the analysis technology for evaluating the effect of medication using living cells. This proposed system consists of two elemental technologies. One is the 3-D component-distribution measurement, another one is the micro-probe operation for local dosing. In this measurement technology, the cell is rotated by light pressure for obtaining the 2-D images from multiple directions. And the 3-D component distribution is reconstructed from these 2-D images. The micro-probe operation that is actuated by light pressure can control the position and attitude of micro-ample. The opposed type beams illuminate the cell at the proximal two points. In this case, the light pressure can act as the rotating torque. And, we can successfully obtain the 3-D phase-contrast images. Furthermore, by operating the distance between two focal points, we can control the attitude of bacillary micro-probe. In addition, we describe the separate measurement of viscous force and elastic force for the cell palpation. This method focuses attention on the surface effect that acts as the dominant factor rather than the volume effect for microscope. Thus, we construct the dynamical model that considers about the viscous-force by the biological cells and the viscous force by the nutrient medium. And these forces are separately calculated from the measured vibration amplitude.

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