Dr. Laura D'Alfonso Profile

Dr. Laura D'Alfonso

Professor at Univ degli Studi di Milano-Bicocca

SPIE Involvement:

Author

Publications (16)

Proceedings Article | 12 August 2023 Presentation

SuperμMAPPS: a supervised AI-phasor-based method to investigate collagen micro-architecture and early detect tumorous tissue in human samples

Riccardo Scodellaro, Davide Panzeri, Elena Pagani, Margaux Bouzin, Laura D'Alfonso, Maddalena Collini, Giuseppe Chirico, Laura Sironi

Proceedings Volume PC12622, PC126220A (2023) https://doi.org/10.1117/12.2673863

KEYWORDS: Tumors, Tissues, Collagen, Biological samples, Cancer detection, Second harmonic generation, Statistical analysis, Signal generators, Visualization, Thyroid

Read Abstract +

Proceedings Article | 12 August 2023 Presentation

Virtually stained H&E images and nuclei segmentation combining neural networks and spectral phasor analysis

Elena Pagani, Davide Panzeri, Riccardo Scodellaro, Margaux Bouzin, Laura D'Alfonso, Maddalena Collini, Giuseppe Chirico, Donato Inverso, Laura Sironi

Proceedings Volume PC12622, PC126220J (2023) https://doi.org/10.1117/12.2673774

KEYWORDS: Image segmentation, Biological research, Neural networks, Nervous system, Microscopes, Tumors, Tissues, Pathology, Liver, Image analysis

Read Abstract +

Proceedings Article | 27 May 2022 Paper

A miniaturized chip for 3D optical imaging of tissue regeneration in vivo

Claudio Conci, Emanuela Jacchetti, Laura Sironi, Lorenzo Gentili, Giulio Cerullo, Rebeca Martinez, Roberto Osellame, Mario Marini, Margaux Bouzin, Maddalena Collini, Laura D'Alfonso, Elmina Kabouraki, Maria Farsari, Anthi Ranella, Nikos Kehagias, Giuseppe Chirico, Manuela Raimondi

Proceedings Volume 12144, 121440D (2022) https://doi.org/10.1117/12.2629824

KEYWORDS: Tissues, Microscopy, In vivo imaging, Confocal microscopy, Two photon polymerization, Tissue optics, Spatial light modulators, Multiphoton fluorescence microscopy

Read Abstract +

Proceedings Article | 20 June 2021 Presentation

Development and characterization of proteinaceous multiphoton-fabricated microstructures with photo-thermal functionality

Mario Marini, Amirbahador Zeynali, Margaux Bouzin, Laura Sironi, Laura D'Alfonso, Piersandro Pallavicini, Maddalena Collini, Giuseppe Chirico

Proceedings Volume 11786, 117861U (2021) https://doi.org/10.1117/12.2593350

KEYWORDS: Nanoparticles, Microfluidics, 3D microstructuring, Tunable lasers, Thermography, Spatial resolution, Proteins, Printing, Neurons, Multiphoton lithography

Read Abstract +

We exploit two-photon laser writing to fabricate 3D biocompatible proteinaceous microstructures (∼1 to 50 𝜇m in lateral size) with tunable elasticity and photo-thermal activity in the near-infrared. Structure printing relies on the photo-crosslinking of the protein bovine serum albumin (BSA, 50 mg/mL) initiated by the Rose Bengal dye (2 mM concentration), whereas photo-thermal functionality is achieved by the dispersion of non-spherically symmetric metallic nanoparticles into the ink. Aiming at a subsequent application of the fabricated microstructures as platforms for cell growth and stimulation, we carry out a thorough characterization of their mechanical and photo-thermal properties. Preliminary data obtained by AFM indentation have quantified the structures Young modulus in the broad 100-1000 kPa range depending on the BSA concentration. Stiffness is further characterized here by subjecting the fabricated microstructures to steady flow in a microfluidic device, and by quantifying their real-time bending by a conventional transmitted light microscope. In parallel, we focus on the optimization of the photo-thermal activity of the structures. Anisotropic gold nanoparticles, dispersed in the ink, get trapped into the structure during photo-crosslinking and lead to localized heat release upon excitation in the near-infrared. The temperature increment is rapidly (∼1 s) reached and maintained under continuous wave laser irradiation at 800 nm; the amplitude of the temperature variation is quantified as a function of the incident laser power by means of infrared thermography and is correlated to both the structure thickness and the nanoparticles concentration. The resulting spatially confined heat loads could be exploited to induce highly localized responses in cells. In this direction, proteinaceous photo-thermal microstructures can be used to physically induce the differentiation of cells (e.g. neurons or fibroblasts) in a spatially controlled manner.

SPIE Journal Paper | 27 February 2019 Open Access

Adaptive optics microspectrometer for cross-correlation measurement of microfluidic flows

Maddalena Collini, Fabrizio Radaelli, Laura Sironi, Nicolo Ceffa, Laura D'Alfonso, Margaux Bouzin, Giuseppe Chirico

JBO, Vol. 24, Issue 02, 025004, (February 2019) https://doi.org/10.1117/12.10.1117/1.JBO.24.2.025004

KEYWORDS: Spatial light modulators, Aberration correction, Adaptive optics, Cameras, Optical aberrations, Wavefronts, Microfluidics, Statistical analysis, Luminescence, Rhodamine

Read Abstract +

DOWNLOAD PAPER

SPIE Journal Paper | 13 October 2017 Open Access

Spatiotemporal image correlation analysis of blood flow in branched vessel networks of zebrafish embryos

Nicolo Ceffa, Ilaria Cesana, Maddalena Collini, Laura D’Alfonso, Silvia Carra, Franco Cotelli, Laura Sironi, Giuseppe Chirico

JBO, Vol. 22, Issue 10, 106008, (October 2017) https://doi.org/10.1117/12.10.1117/1.JBO.22.10.106008

KEYWORDS: Image analysis, Blood circulation, Blood, Imaging spectroscopy, Capillaries, In vivo imaging, Spatial resolution, Spectroscopy, Objectives, Microfluidics

Read Abstract +

DOWNLOAD PAPER

Proceedings Article | 26 June 2017 Paper

Scanless nonlinear optical microscope for image reconstruction and space-time correlation analysis

N. Ceffa, F. Radaelli, P. Pozzi, M. Collini, L. Sironi, L. D'alfonso, G. Chirico

Proceedings Volume 10333, 103330E (2017) https://doi.org/10.1117/12.2271832

KEYWORDS: Spatial light modulators, Microscopes, Charge-coupled devices, Image analysis, Image acquisition, Tissues, Blood circulation, Tissue optics, Nonlinear optics, Microscopy, Luminescence, Optical microscopes, Image restoration, Biological research, Modulators, Fluorescence spectroscopy, Spectroscopy

Read Abstract +

Proceedings Article | 27 April 2016 Paper

Gold nanocages for imaging and therapy of prostate cancer cells

Laura Sironi, Svetlana Avvakumova, Elisabetta Galbiati, Silvia Locarno, Chiara Macchi, Laura D'Alfonso, Massimiliano Ruscica, Paolo Magni, Maddalena Collini, Sergio Romeo, Giuseppe Chirico, Davide Prosperi

Proceedings Volume 9887, 988721 (2016) https://doi.org/10.1117/12.2229715

KEYWORDS: Prostate cancer, Gold, Biomedical optics, Confocal microscopy, Cancer, Prostate cancer, Cancer, Luminescence, Nanoparticles, Near infrared, Pulsed laser operation, Tissues, Microscopy

Read Abstract +

Proceedings Article | 22 June 2015 Paper

Single image correlation for blood flow mapping in complex vessel networks

Giuseppe Chirico, Laura Sironi, Margaux Bouzin, Laura D'Alfonso, Maddalena Collini, Nicolo’ Ceffa, Cassia Marquezin

Proceedings Volume 9529, 95290F (2015) https://doi.org/10.1117/12.2190739

KEYWORDS: Blood circulation, Confocal microscopy, Luminescence, Diffusion, Capillaries, Image analysis, Hemodynamics, Tissues, Spatial resolution, Fluorescence correlation spectroscopy

Read Abstract +

Microcirculation plays a key role in the maintenance and hemodynamics of tissues and organs also due to its extensive interaction with the immune system. A critical limitation of state-of-the-art clinical techniques to characterize the blood flow is their lack of the spatial resolution required to scale down to individual capillaries. On the other hand the study of the blood flow through auto- or cross-correlation methods fail to correlate the flow speed values with the morphological details required to describe an intricate network of capillaries. Here we propose to use a newly developed technique (FLICS, FLow Image Correlation Spectroscopy) that, by employing a single raster-scanned xy-image acquired in vivo by confocal or multi-photon excitation fluorescence microscopy, allows the quantitative measurement of the blood flow velocity in the whole vessel pattern within the field of view, while simultaneously maintaining the morphological information on the immobile structures of the explored circulatory system. Fluorescent flowing objects produce diagonal lines in the raster-scanned image superimposed to static morphological details. The flow velocity is obtained by computing the Cross Correlation Function (CCF) of the intensity fluctuations detected in pairs of columns of the image. The whole analytical dependence of the CCFs on the flow speed amplitude and the flow direction has been reported recently. We report here the derivation of approximated analytical relations that allows to use the CCF peak lag time and the corresponding CCF value, to directly estimate the flow speed amplitude and the flow direction. The validation has been performed on Zebrafish embryos for which the flow direction was changed systematically by rotating the embryos on the microscope stage. The results indicate that also from the CCF peak lag time it is possible to recover the flow speed amplitude within 13% of uncertainty (overestimation) in a wide range of angles between the flow and the image scanning direction.

Proceedings Article | 5 March 2015 Paper

Fluorescence cross-correlation spectroscopy for time dependent flows: a numerical investigation

Nicolo' Ceffa, Paolo Pozzi, Margaux Bouzin, Cassia Marquezin, Laura Sironi, Laura D'Alfonso, Maddalena Collini, Giuseppe Chirico

Proceedings Volume 9320, 93200R (2015) https://doi.org/10.1117/12.2077088

KEYWORDS: Modulation, Capillaries, Luminescence, Diffusion, Blood, Blood circulation, Microfluidics, Fluorescence spectroscopy, Spectroscopy, Numerical analysis

Read Abstract +

SPIE Journal Paper | 20 June 2014 Open Access

Electron multiplying charge-coupled device-based fluorescence cross-correlation spectroscopy for blood velocimetry on zebrafish embryos

Paolo Pozzi, Laura Sironi, Laura D’Alfonso, Margaux Bouzin, Maddalena Collini, Giuseppe Chirico, Piersandro Pallavicini, Franco Cotelli, Efrem Foglia

JBO, Vol. 19, Issue 06, 067007, (June 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.6.067007

KEYWORDS: Diffusion, Capillaries, Luminescence, Blood, Veins, Blood circulation, Solids, Fluorescence spectroscopy, Spectroscopes, Arteries

Read Abstract +

DOWNLOAD PAPER

Proceedings Article | 1 March 2013 Paper

Structured illumination fluorescence correlation spectroscopy for velocimetry in Zebrafish embryos

Paolo Pozzi, Leone Rossetti, Laura Sironi, Stefano Freddi, Laura D'Alfonso, Michele Caccia, Margaux Bouzin, Maddalena Collini, Giuseppe Chirico

Proceedings Volume 8580, 85800V (2013) https://doi.org/10.1117/12.2002082

KEYWORDS: Diffusion, Luminescence, Blood, Veins, Fluorescence correlation spectroscopy, Gold, Solids, Rhodamine, Signal detection, Nanorods

Read Abstract +

Proceedings Article | 13 February 2010 Paper

In-vitro and in-vivo detection of p53 by fluorescence lifetime on a hybrid FITC-gold nanosensor

L. Sironi, S. Freddi, L. D'Alfonso, M. Collini, T. Gorletta, S. Soddu, G. Chirico

Proceedings Volume 7574, 757403 (2010) https://doi.org/10.1117/12.841094

KEYWORDS: Gold, Proteins, Luminescence, In vitro testing, Technetium, In vivo imaging, Tumors, Nanoparticles, Nanosensors, Cancer

Read Abstract +

Proceedings Article | 9 October 2003 Paper

Novel efficient and stable heteroaromatic two-photon absorbing dyes

Alessandro Abbotto, Luca Beverina, Giorgio Pagani, Maddalena Collini, Giuseppe Chirico, Laura D'Alfonso, Giancarlo Baldini

Proceedings Volume 5139, (2003) https://doi.org/10.1117/12.500564

KEYWORDS: Absorption, Chromophores, Nitrogen, Luminescence, Molecules, Near infrared, Optical properties, Biomedical optics, Visible radiation, Quantum efficiency

Read Abstract +

Proceedings Article | 9 October 2003 Paper

Confocal microscopy: an experimental set up for biomolecule structure investigation based on dynamical fluorescence spectroscopy

Amanda Oldani, Maddalena Collini, Giuseppe Chirico, Laura D'Alfonso, Giancarlo Baldini, Filomena Mazzei, Flavia Barone, Luciano Cellai, Cesare Giordano, Marziale Milani, Antonio Pasinetti

Proceedings Volume 5139, (2003) https://doi.org/10.1117/12.500197

KEYWORDS: Modulation, Microscopes, Phase shift keying, Luminescence, Anisotropy, Fluorescence anisotropy, Confocal microscopy, Fluorometers, Polarization, Objectives

Read Abstract +

Proceedings Article | 9 October 2003 Paper

New two-photon excitation chromophores for cellular imaging

Laura D'Alfonso, Giuseppe Chirico, Maddalena Collini, Giancarlo Baldini, Alberto Diaspro, Paola Ramoino, Alessandro Abbotto, Luca Beverina, Giorgio Pagani

Proceedings Volume 5139, (2003) https://doi.org/10.1117/12.500637

KEYWORDS: Luminescence, Fluorescence correlation spectroscopy, Two photon excitation microscopy, Bioalcohols, Confocal microscopy, Absorption, Rhodamine, Yeast, Proteins, Quantum efficiency

Read Abstract +

Showing 5 of 16 publications

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Keywords/Phrases

Search In:

Publication Years