KEYWORDS: Fluorescence resonance energy transfer, Luminescence, Laser irradiation, Proteins, Fluorescent proteins, Kidney, Life sciences, Cell mechanics, Molecules, Molecular energy transfer
Low-power laser irradiation (LPLI) has been regarded as playing a significant role in triggering cellular survival and
proliferation. However, the mechanism has not been fully understood. In this study, using real-time single-cell analysis,
we investigated the activity of Akt and its effects on cell proliferation induced by LPLI in african green monkey
SV40-transformed kidney fibroblast cells (COS-7). We utilized a recombinant fluorescence resonance energy transfer
(FRET) Akt probe (BKAR) to dynamically detect the activation of Akt after LPLI treatment. Our results show that
LPLI induced a gradual and continuous activation of Akt. Moreover, the activation of Akt can be completely abolished
by wortmannin, a specific inhibitor of PI3K, suggesting that the activation of Akt caused by LPLI is a PI3K-dependent
event. LPLI promotes cell proliferation through Akt activation since the cell viability was significantly inhibited by PI3K
inhibitor. We thus conclude that, Akt activation is well involved in LPLI triggered cell proliferation that acts as a time and dose-dependent manner.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.