Dr. Lukas Kontenis Profile

Dr. Lukas Kontenis

R&D Engineer at Light Conversion, UAB

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Publications (11)

Proceedings Article | 13 March 2024 Presentation

Polarimetric third-harmonic generation microscopy for histopathology

Mykolas Maciulis, Viktoras Mazeika, Vilte Samsone, Lukas Kontenis, Edvardas Zurauskas, Vitalijus Karabanovas, Virginijus Barzda

Proceedings Volume PC12846, PC128460D (2024) https://doi.org/10.1117/12.3001641

KEYWORDS: Third harmonic generation, Second harmonic generation, Polarimetry, Microscopy, Histopathology, Visualization, Tissues, Image visualization, Connective tissue, Collagen

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Proceedings Article | 13 March 2024 Presentation

High power and energy tunable lasers for 2-photon imaging

Marco Arrigoni, Jonas Berzinš, Lukas Kontenis, Mantvydas Mikulis

Proceedings Volume PC12847, PC1284706 (2024) https://doi.org/10.1117/12.3014131

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Proceedings Article | 13 March 2024 Presentation

Development of turn-key lasers for three-photon microscopy

Jonas Berzinš, Mantvydas Mikulis, Lukas Kontenis

Proceedings Volume PC12847, PC128470A (2024) https://doi.org/10.1117/12.3003079

KEYWORDS: Laser development, Multiphoton microscopy, Optical parametric amplifiers, Neuroscience, Imaging systems, Biomedical optics, Biomedical applications, Reliability, Optical benches, Microscopy

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Proceedings Article | 13 March 2024 Presentation

Origin of collagen chiral response in polarimetric second harmonic generation microscopy

Mehdi Alizadeh, Viktoras Mazeika, Danute Bulotiene, Vitalijus Karabanovas, Lukas Kontenis, Virginijus Barzda

Proceedings Volume PC12847, PC128470O (2024) https://doi.org/10.1117/12.3003060

KEYWORDS: Collagen, Second harmonic generation, Polarimetry, Microscopy, Laser radiation, Visualization, Statistical analysis, Diseases and disorders, Dichroic materials

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Proceedings Article | 13 March 2024 Presentation

Disorder in collagen at the tumor boundary investigated with double Stokes-Mueller polarimetric microscopy

Viktoras Mažeika, Mykolas Mačiulis, Lukas Kontenis, Mehdi Alizadeh, Masood Samim, Serguei Krouglov, Edvardas Žurauskas, Virginijus Barzda

Proceedings Volume PC12854, PC128540J (2024) https://doi.org/10.1117/12.3002404

KEYWORDS: Polarimetry, Collagen, Diseases and disorders, Tumors, Second harmonic generation, Cooccurrence matrices, Nonlinear susceptibility, Mueller matrices, Tissues, Polarization

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Proceedings Article | 17 March 2023 Poster

We need to talk about laser pulse energy stability

Lukas Kontenis, Mikas Urbšas, Jonas Berzinš, Karolis Neimontas

Proceedings Volume PC12384, PC1238410 (2023) https://doi.org/10.1117/12.2649932

KEYWORDS: Pulsed laser operation, Laser stabilization, Laser energy, Laser development, Signal to noise ratio, Sensors, Multiphoton microscopy, Laser sources, Image acquisition, Femtosecond phenomena

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Proceedings Article | 30 May 2022 Presentation

Investigation of tumor extracellular matrix using polarimetric second-harmonic generation microscopy and texture analysis

Viktoras Mažeika, Mykolas Maciulis, Lukas Kontenis, Edvardas Zurauskas, Martynas Riauka, Mehdi Alizadeh, Kamdin Mirsanaye, Virginijus Barzda

Proceedings Volume PC12144, PC121440R (2022) https://doi.org/10.1117/12.2621392

KEYWORDS: Polarimetry, Second-harmonic generation, Tumors, Microscopy, Collagen, Polarization, Signal detection, Cancer, Pancreas, Microscopes

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Extracellular matrix (ECM) has important functions in cell proliferation, differentiation, and migration, which influence the development and progression of cancer. ECM in tumor microenvironment experiences changes in composition and structure that can appear early in tumor development and could serve as a biomarker for cancer diagnostics. In addition, some changes in ECM may correlate with the rate of tumor progression or its tendency to form metastases and would allow to predict future tumor development [1]. Collagen is an important structural protein found in ECM. It has a non-centrosymmetric structure, and, thus, can be easily visualized using second harmonic generation (SHG) microscopy. SHG microscopy employs certain polarimetric techniques to gain detailed information about the organization of collagen in various tissues [2]. In this work, polarimetric SHG microscopy is used to acquire collagen images from normal and cancerous regions of human colon and pancreas histological samples. Texture analysis is performed on SHG intensity and polarization images to characterize the distribution of ultrastructure parameters in the tissue. Significant differences are observed in collagen ultrastructure between normal and tumor areas. Further, collagen structures of colon and pancreas tumor microenvironments are compared to investigate relative differences in ECM organization between the tissues. Finally, a machine learning classifier is used to group the acquired images in tumor and normal groups. The results show potential for development of novel cancer diagnostic technique using polarimetric second harmonic generation microscopy and texture analysis. [1] Winkler, J. et al., “Concepts of extracellular matrix remodelling in tumour progression and metastasis”, Nat Commun 11, 5120 (2020). [2] Golaraei, A. et al., “Polarimetric second-harmonic generation microscopy of the hierarchical structure of collagen in stage I-III non-small cell lung carcinoma,” Biomed. Opt. Express 11, 1851-1863 (2020).

Proceedings Article | 7 December 2021 Paper

Nonlinear multicontrast microscopy with texture analysis for colon cancer diagnostics

Viktoras Mažeika, Mykolas Mačiulis, Martynas Riauka, Lukas Kontenis, Edvardas Žurauskas, Virginijus Barzda

Proceedings Volume 11919, 1191913 (2021) https://doi.org/10.1117/12.2614800

KEYWORDS: Second-harmonic generation, Microscopy, Statistical analysis, Analytical research, Tissues, Polarimetry, Diagnostics, Colorectal cancer, Colon, Collagen

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Proceedings Article | 7 December 2021 Paper

Wide-field polarization-resolved SHG microscopy in biomedical imaging

Kamdin Mirsanaye, Leonardo Uribe Castano, Yasmeen Kamaliddin, Ahmad Golaraei, Lukas Kontenis, Susan Done, Edvardas Žurauskas, Brian Wilson, Virginijus Barzda

Proceedings Volume 11922, 119220M (2021) https://doi.org/10.1117/12.2615714

KEYWORDS: Tissues, Second-harmonic generation, Microscopy, Collagen, Tumors, Cancer, Biomedical optics, Polarization, Polarimetry, Diagnostics

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Proceedings Article | 3 December 2021 Paper

Characterization of lentiginous melanoma tissue with multimodal polarimetric harmonic generation microscopy

Martynas Riauka, Viktoras Mazeika, Mykolas Maciulis, Edvardas Zurauskas, Lukas Kontenis, Virginijus Barzda

Proceedings Volume 11921, 1192104 (2021) https://doi.org/10.1117/12.2614313

KEYWORDS: Tissues, Polarimetry, Collagen, Second-harmonic generation, Polarization, Microscopy, Melanoma, Harmonic generation, Polysomnography, Cancer

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Proceedings Article | 28 July 2017 Paper

Experimental implementation of THG Stokes-Mueller polarimetric microscopy

Lukas Kontenis, Masood Samim, Virginijus Barzda

Proceedings Volume 10414, 1041404 (2017) https://doi.org/10.1117/12.2284165

KEYWORDS: Polarimetry, Microscopy, Third-harmonic generation

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Showing 5 of 11 publications

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