Dr. Mahsa Ranji Profile

Dr. Mahsa Ranji

Faculty at Florida Atlantic Univ

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Author

Publications (19)

SPIE Journal Paper | 8 July 2021 Open Access

Three-dimensional vascular and metabolic imaging using inverted autofluorescence

Shima Mehrvar, Soudeh Mostaghimi, Amadou K. Camara, Farnaz Foomani, Jayashree Narayanan, Brian Fish, Meetha Medhora, Mahsa Ranji

JBO, Vol. 26, Issue 07, 076002, (July 2021) https://doi.org/10.1117/12.10.1117/1.JBO.26.7.076002

KEYWORDS: Image segmentation, 3D image processing, Kidney, Luminescence, Lung, Video, Liver, Tissues, 3D modeling, Heart

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Proceedings Article | 20 February 2018 Paper

Optical cryoimaging for assessment of radiation-induced injury to rat kidney metabolic state

Shima Mehrvar, Mette Funding la Cour, Meetha Medhora, Amadou K. Camara, Mahsa Ranji

Proceedings Volume 10497, 1049709 (2018) https://doi.org/10.1117/12.2291555

KEYWORDS: Kidney, Tissues, Injuries, Luminescence, Control systems, 3D image processing, Tissue optics, Biomedical optics, Oxidation, Image processing

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SPIE Journal Paper | 19 January 2018 Open Access

Optical metabolic imaging of irradiated rat heart exposed to ischemia–reperfusion injury

Mette Funding la Cour, Shima Mehrvar, James Heisner, Mohammad Masoudi Motlagh, Meetha Medhora, Mahsa Ranji, Amadou K. Camara

JBO, Vol. 23, Issue 01, 016011, (January 2018) https://doi.org/10.1117/12.10.1117/1.JBO.23.1.016011

KEYWORDS: Heart, Ischemia, Injuries, Technetium, Optical imaging, Control systems, Infrared imaging, Tissues, Acquisition tracking and pointing, Optical filters

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Whole thoracic irradiation (WTI) is known to cause deterioration in cardiac function. Whether irradiation predisposes the heart to further ischemia and reperfusion (IR) injury is not well known. The aim of this study is to examine the susceptibility of rat hearts to IR injury following a single fraction of 15 Gy WTI and to investigate the role of mitochondrial metabolism in the differential susceptibility to IR injury. After day 35 of irradiation, ex vivo hearts from irradiated and nonirradiated rats (controls) were exposed to 25-min global ischemia followed by 60-min IR, or hearts were perfused without IR for the same protocol duration [time controls (TC)]. Online fluorometry of metabolic indices [redox state: reduced nicotinamide adenine dinucleotide (NADH), oxidized flavin adenine dinucleotide (FAD), and NADH/FAD redox ratio] and functional variables [systolic left ventricular pressure (LVP), diastolic LVP (diaLVP), coronary flow (CF), and heart rate were recorded in the beating heart; developed LVP (dLVP) and rate pressure product (RPP)] were derived. At the end of each experimental protocol, hearts were immediately snap frozen in liquid N2 for later three-dimensional imaging of the mitochondrial redox state using optical cryoimaging. Irradiation caused a delay in recovery of dLVP and RPP after IR when compared to nonirradiated hearts but recovered to the same level at the end of reperfusion. CF in the irradiated hearts recovered better than the control hearts after IR injury. Both fluorometry and 3-D cryoimaging showed that in WTI and control hearts, the redox ratio increased during ischemia (reduced) and decreased on reperfusion (oxidized) when compared to their respective TCs; however, there was no significant difference in the redox state between WTI and controls. In conclusion, our results show that although irradiation of rat hearts compromised baseline cardiovascular function, it did not alter cardiac mitochondrial redox state and induce greater susceptibility of these hearts to IR injury.

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Proceedings Article | 16 February 2017 Paper

Time-lapse microscopy of lung endothelial cells under hypoxia

Shima Mehrvar, Zahra Ghanian, Ganesh Kondouri, Amadou Camara, Mahsa Ranji

Proceedings Volume 10068, 1006822 (2017) https://doi.org/10.1117/12.2254866

KEYWORDS: Fetus, Time lapse microscopy, Hypoxia, Oxygen, Lung, Injuries, Image segmentation

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Proceedings Article | 26 April 2016 Presentation

Optical imaging of irradiated and non-irradiated hearts (Conference Presentation)

Stephanie Bolin, Guanchu Chen, Meetha Medhora, Amadou K. Camara, Mahsa Ranji

Proceedings Volume 9706, 97061G (2016) https://doi.org/10.1117/12.2213327

KEYWORDS: Heart, Ischemia, Infrared imaging, Injuries, Tissues, Optical imaging, Infrared radiation, 3D image processing, Tissue optics, Infrared materials

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Objective: In this study, the metabolic state of the heart tissue is studied in a rodent model of ischemia and reperfusion (IR) in rats exposed to irradiation injury using a cryofluorescence imaging technique. Mitochondrial metabolic state is evaluated by autofluorescence of mitochondrial metabolic coenzymes NADH and FAD. The redox ratio (NADH/FAD) is used as a biochemical/metabolic marker of oxidative stress, before, during and after IR. Materials and methods: Hearts were extracted from non-irradiated (control) and irradiated rats (Irr) given 15 Gy whole thorax irradiation rats (WTI). After 35 days, before the onset of radiation pneumonitis, these two groups of hearts were subjected to one of three treatments; Time control (TC; hearts perfused for the duration of the protocol without ischemia or IR), 25 minutes ischemia with no reperfusion and 25 minutes ischemia followed by 60 minutes reperfusion (IR). Hearts were removed from the Langendorff perfusion system and immediately snap frozen in liquid N2 to preserve the metabolic state after injury; 3-dimensional (3D) cryo-fluorescent imager was used to obtain in fixed time NADH and FAD fluorescence images and their distribution across the entire ventricles. In this study, a 30-μm axial resolution was used resulting in 550 cross-section images per heart. The 3D images of the redox ratio and their respective histograms were calculated in the six groups of hearts. Results: We compared the mean values of the redox ratio in each group, which demonstrate a reduced mitochondrial redox state in both irradiated and non-irradiated ischemic hearts and an oxidized mitochondrial redox state for both irradiated and non-irradiated ischemia-reperfusion hearts compared to control hearts. For non-irradiated hearts, ischemia and IR injuries resulted respectively in 61% increase and 54% decrease in redox ratio when compared with TC. For irradiated hearts, ischemia and IR injuries resulted respectively in 90% increase and 50% decrease in redox ratio when compared to TC. Conclusion: The cryoimager is able to quantify ischemia and IR injuries. Cryoimaging is a unique 3D imaging tools that provides quantitative measurement of tissue metabolic state. Hearts that underwent irradiation indicates a more oxidized metabolic state in the tissue. This change persists across all three treatments.

Proceedings Article | 5 March 2015 Paper

Optical cryoimaging of rat kidney and the effective role of chromosome 13 in salt-induced hypertension

F. Salehpour, Chun Yang, Theresa Kurth, A. Cowley, M. Ranji

Proceedings Volume 9321, 932113 (2015) https://doi.org/10.1117/12.2079549

KEYWORDS: Kidney, Tissues, Tissue optics, Image segmentation, 3D modeling, Injuries, 3D image processing, Genetics, Optical imaging, Signal detection

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Proceedings Article | 2 March 2015 Paper

Optical Studies of Oxidative Stress in Pulmonary Artery Endothelial Cells

Zahra Ghanian, Reyhaneh Sepehr, Annie Eis, Ganesh Kondouri, Mahsa Ranji

Proceedings Volume 9328, 932807 (2015) https://doi.org/10.1117/12.2076658

KEYWORDS: Oxygen, Injuries, Arteries, Potassium, Cyanide, Luminescence, Signal processing, Microscopy, Electron transport, Fetus

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Proceedings Article | 4 March 2014 Paper

Cytometric analysis of retinopathies in retinal trypsin digests

Zahra Ghanian, Kevin Staniszewski, Christine Sorenson, Nader Sheibani, Mahsa Ranji

Proceedings Volume 8947, 89471Y (2014) https://doi.org/10.1117/12.2036852

KEYWORDS: Retina, Capillaries, Image segmentation, Feature extraction, Luminescence, Cell death, Error analysis, Injuries, Binary data, Medicine

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Proceedings Article | 1 March 2013 Paper

Fluorometry of ischemia reperfusion injury in rat lungs in vivo

R. Sepehr, K. Staniszewski, E. Jacobs, S. Audi, Mahsa Ranji

Proceedings Volume 8580, 85800W (2013) https://doi.org/10.1117/12.2002094

KEYWORDS: Lung, Luminescence, Tissue optics, Ischemia, In vivo imaging, Injuries, Tissues, Blood, Fluorometers, Reflectivity

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Proceedings Article | 25 February 2013 Paper

Optical imaging of oxidative stress in retinitis pigmentosa (RP) in rodent model

Zahra Ghanian, Sepideh Maleki, Sandeep Gopalakrishnan, Reyhaneh Sepehr, Janis Eells, Mahsa Ranji

Proceedings Volume 8591, 85910S (2013) https://doi.org/10.1117/12.2004843

KEYWORDS: Near infrared, Retina, Eye, Tissues, Eye models, Tissue optics, Optical imaging, Animal model studies, Luminescence, Image processing

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SPIE Journal Paper | 4 January 2013 Open Access

Optical imaging of mitochondrial redox state in rodent model of retinitis pigmentosa

Sepideh Maleki, Sandeep Gopalakrishnan, Zahra Ghanian, Reyhaneh Sepehr, Heather Schmitt, Janis Eells, Mahsa Ranji

JBO, Vol. 18, Issue 01, 016004, (January 2013) https://doi.org/10.1117/12.10.1117/1.JBO.18.1.016004

KEYWORDS: Retina, Tissue optics, Tissues, Eye, Optical imaging, Oxygen, Luminescence, Eye models, In vivo imaging, 3D image processing

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SPIE Journal Paper | 19 April 2012 Open Access

Optical imaging of tissue mitochondrial redox state in intact rat lungs in two models of pulmonary oxidative stress

Reyhaneh Sepehr, Kevin Staniszewski, Sepideh Maleki, Mahsa Ranji, Elizabeth Jacobs, Said Audi

JBO, Vol. 17, Issue 04, 046010, (April 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.4.046010

KEYWORDS: Lung, Tissues, Injuries, Tissue optics, Luminescence, Hyperoxia, Optical imaging, Blood, 3D image processing, Infrared imaging

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Proceedings Article | 9 February 2012 Paper

Optical cryo-imaging of kidney mitochondrial redox state in diabetic mice models

S. Maleki, R. Sepehr, K. Staniszewski, N. Sheibani, C. Sorenson, M. Ranji

Proceedings Volume 8225, 82252A (2012) https://doi.org/10.1117/12.909394

KEYWORDS: Kidney, Tissues, Luminescence, Tissue optics, Mouse models, Medicine, Oxygen, Proteins, Optical imaging, Cancer

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Proceedings Article | 9 February 2012 Paper

Optical studies of tissue mitochondrial redox in isolated perfused rat lungs

R. Sepehr, K. Staniszewski, E. Jacobs, S. Audi, M. Ranji

Proceedings Volume 8207, 82073Q (2012) https://doi.org/10.1117/12.909474

KEYWORDS: Lung, Tissues, Natural surfaces, Luminescence, Tissue optics, Fluorometers, Potassium, Optical instrument design, Fiber optics, Electronic filtering

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Proceedings Article | 9 February 2012 Paper

Classification of retinopathic injury using image cytometry and vasculature complexity

K. Staniszewski, R. Sepehr, C. Sorenson, N. Sheibani, M. Ranji

Proceedings Volume 8225, 822526 (2012) https://doi.org/10.1117/12.908598

KEYWORDS: Retina, Injuries, Image classification, Luminescence, Image segmentation, Cell death, Feature extraction, Classification systems, Optical filters, Blood vessels

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Proceedings Article | 9 June 2011 Paper

Fluorescence spectroscopy and cryoimaging of rat lung tissue mitochondrial redox state

R. Sepehr, S. Audi, K. Staniszewski, S. Maleki, M. Ranji

Proceedings Volume 8087, 80870A (2011) https://doi.org/10.1117/12.890019

KEYWORDS: Lung, Tissues, Luminescence, Tissue optics, Natural surfaces, Potassium, Fluorescence spectroscopy, Biomedical optics, Fluorometers, Cyanide

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Proceedings Article | 13 February 2007 Paper

Fluorescence spectroscopy to assess apoptosis in myocardium

Mahsa Ranji, Muneaki Matsubara, Michael Grosso, Dwight Jaggard, Britton Chance, Robert Gorman, Joseph Gorman

Proceedings Volume 6438, 64380J (2007) https://doi.org/10.1117/12.700399

KEYWORDS: Cell death, Tissue optics, Ischemia, Injuries, Luminescence, Heart, Fluorescence spectroscopy, In vivo imaging, Fluorometers, Signal detection

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SPIE Journal Paper | 1 November 2006 Open Access

Fluorescence spectroscopy and imaging of myocardial apoptosis

Mahsa Ranji, Shinya Kanemoto, Muneaki Matsubara, Michael Grosso, Joseph Gorman, Robert Gorman, Dwight Jaggard, Britton Chance

JBO, Vol. 11, Issue 06, 064036, (November 2006) https://doi.org/10.1117/12.10.1117/1.2400701

KEYWORDS: Cell death, Heart, Luminescence, Imaging spectroscopy, Signal detection, In vivo imaging, Ischemia, Tissue optics, Fluorescence spectroscopy, Tissues

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Proceedings Article | 24 February 2006 Paper

Observation of mitochondrial morphology and biochemistry changes undergoing apoptosis by angularly resolved light scattering and cryoimaging

Mahsa Ranji, Dwight Jaggard, Britton Chance

Proceedings Volume 6087, 60870K (2006) https://doi.org/10.1117/12.646255

KEYWORDS: Cell death, Light scattering, Mie scattering, Mode conditioning cables, Luminescence, Electron microscopy, Scattering, Tissues, Tissue optics, Optical fibers

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Showing 5 of 19 publications

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