The Mycobacterium tuberculosis (MbT) drug resistance remains a serious problem for global public health and usually leads to a lethal outcome. Precise and rapid identification of a specific MbT strain is vital for the appointment of antituberculosis therapy. We continue investigation of deactivated MbT strains of the Beijing family by Raman spectroscopy method and focus on Raman spectra analysis for the identification of potential biomarkers for different MbT strains. . We have studied extrapulmonary Beijing strain with XDR resistance. Samples were investigated by Raman spectrometry with He-Ne (λ=633 nm) laser excitation source in the «fingerprint» region. As a result, new spectral features were determined. They describe spectral discrimination between drug-sensitive and drug-resistant strains identified for extra-pulmonary forms of tuberculosis. We suppose that the obtained results can be useful for new diagnostic tools development for the identification of different MbT strains in clinical and research practice.
The rezazurin assay for screening of bacterial growth remains the most popular method for express diagnostic tests. It is based on the change of colour and uorescence properties of the respective dye due to bacterial breathing but its conventional quantitative practical implementation requires a relative expensive and cumbersome spectrophotometric equipment. As an alternative approach, we propose to shift a focus from hardware to software. Preliminary results announced in this work show that the processing RGB colour channels for simple photographic images of resazurin assay plates with mycobacterium tuberculosis provides an opportunity to reconstruct the target growth curve with an accuracy comparable with results obtained using the conventional photometric uorescence curves.
We have developed a methodology for the study of deactivated strains of Mycobacterium tuberculosis. Strains of the Beijing species obtained from pulmonary patient secrete (XDR strain) and reference strain (H37Rv) were investigated by Raman spectrometry with He-Ne (632,8 nm) laser excitation source. As a result of the research, the optimal experimental parameters have been obtained to get spectra of mycolic acids, which are part of the cell wall of mycobacteria.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.