In this work we introduce the first step toward the development of an Infrared (IR) sensoristic platform aiming to the detection and discrimination of airborne pathogens. SARS-CoV-2 Spike glycoprotein (S) is considered as a biomarker for virus recognition. A comparative spectroscopic analysis is illustrated, studying Spike glycoprotein subunit S1 from three different viruses, MERS-CoV, SARS-CoV and SARS-CoV-2. Moreover, SARS-CoV-2 variants are also investigated. The IR characterization of their S1 secondary conformation was carried out through Attenuated Total Reflection (ATR), analyzing the Amide I absorption band. In addition, pH-dependent conformational changes of SARS-CoV-2 S1 were investigated, too
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