Digital holographic microscopy (DHM) is an interferometry-based variant of quantitative phase microscopy (QPM) that can be integrated modular into various common microscopes for label-free imaging of fast cellular morphology changes in a biomedical laboratory environment. We have utilized a fiber optics-based off-axis DHM concept to monitor the dynamics of beating cardiomyocytes after drug treatment. Our results show that local height changes due to cellular contractions can be detected spatially resolved at the subcellular level. Moreover, we demonstrate that a temporal resolution in the millisecond range is sufficient to detect drug induced increases and decreases of the beating rate.
Cristina Rusu, Ronny van't Oever, Meint de Boer, Henri Jansen, Erwin Berenschot, Miko Elwenspoek, Martin Bennink, Johannes Kanger, Bart de Grooth, Jan Greve, Juergen Brugger, Albert van den Berg
We have developed a micromachined flow cell consisting of a flow channel integrated with micropipettes. The flow cell is used in combination with an optical trap set-up (optical tweezers) to study mechanical and structural properties of (lambda) -DNA molecules. The flow cell was realized using silicon micromachining including the so-called buried channel technology to fabricate the micropipettes, the wet etching of glass to create the flow channel, and the powder blasting of glass to create the fluid connections. The volume of the flow cell is 2 (mu) l. The pipettes have a length of 130 micrometer, a width of 5 - 10 micrometer, a round opening of 1 micron and can be processed with different shapes. Using this flow cell we stretched single molecules ((lambda) -DNA) showing typical force-extension curves also found with conventional techniques.
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