A MHz akinetic swept source is demonstrated by incorporating a commercial Erbium Doped Fiber Amplifier into a fiber laser cavity assembly. Such laser is brought into mode-locking based on nonlinear polarization rotation (NPR). After the laser, a dispersion compensating fiber is employed to time-stretch the broadband pulses obtained. Different cavity lengths are evaluated, leading to sweeping rates from 0.9 MHz to 9.65 MHz. A maximum of 67 nm bandwidth has been achieved. The swept source has been characterized using a Master-Slave procedure, obtaining an almost linear sweep.
The routine pathology workflow relies on cutting tissue into single-cell layer thick slices using paraffin or frozen sectioning. We propose a fast method to obtain sections of equivalent quality optically using the strong sectioning capabilities of two-photon microscopy (TPM). Hematoxylin and eosin (HE) equivalent staining of the tissue is achieved using acridine orange and sulforhodamine 101. We improved our previously presented pulsed fiber laser to deliver adjustable pulse durations of ~30ps at repetition rates of up to 16MHz and kW peak power. We can now image up to one square centimeter of tissue with sub-micrometer resolution within 15 minutes.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.