A novel on-chip chemiluminescence biosensor is developed to assess cleanliness of the solid surfaces. The sensor is based on ATP-mediated chemiluminescence detection. It provides high-sensitivity detection of surface contamination due to improved photon collection efficiency. The results show the dependence of the bioluminescence enhancement on ATP concentration. We report the limit of detection of the biosensor. We demonstrate the use of the biosensor for real sample analysis to assess surface contamination of the laboratory and office equipment. This suggests the use of the biosensor in practical applications such as the food processing industry, laboratory environments, and health care settings.
SCOBY is a biomaterial derived from a symbiotic culture of bacteria and yeast. Its structure is characterized by intercalated nanocellulose fibers. There has been increased interest in using this bacterial nanocellulose to medical applications and in biosensors. For successful applications, their mechanical, optical, and electrical properties need to be characterized and improved. We report experimental results on the optical absorption, emission, and scattering properties of SCOBY and derivations of SCOBY. In addition, we demonstrate improved electrical conductivity by the addition of PEDOT:PSS and AgNPs into the nanocellulose matrix, leading to the possibility of SERS and luminescence emission for nanoscale biosensing applications.
Extracellular vesicles (EVs) carry molecular cargo that includes nucleic acids, lipids, proteins, and various other biomarkers. Raman and SERS spectroscopy are label-free spectroscopy techniques based on inelastic scattering of laser light interacting with molecular vibrations. In our study, we employed Raman and SERS spectroscopy for the detection of amyloid beta protein in the molecular cargo of small EVs and bulk chemical analysis of EVs. We observed considerable variation as a reflection of the biochemical content of EVs related to the Aβ peptide incorporated in EVs extracted from the AD cell culture model. Next, we developed a new CMOS-based sensing platform for trapping, imaging, and chemical characterization of EVs via SERS (CMOS TrICC) with the experimental enhancement factor 5.0 × 104. We employed this platform for parallel trapping and sensitive biochemical analysis of the 100 nm nanospheres and EVs.
Here we describe a lens free on-sensor imaging platform which is able to perform a high spatial resolution imaging over the large field of view. By measuring the geometrical shadow of the microscopic objects at different oblique illumination angles, we were able to measure the height of these objects. In addition, we were able to record bacterial bioluminescence signals directly on the CMOS chip. This is, to our knowledge, the first report of lens-less, on-sensor bioluminescence measurements.
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