Spatio-Temporal Optical Coherence Tomography (STOC-T) is a new imaging modality that uses light with controlled spatial and temporal coherence. The retinal images acquired with the STOC-T system maintain high resolution in all three dimensions, over the entire thickness of about 700 μm, without mechanical scanning. We have employed known data processing algorithms and developed new ones to handle and process the acquired data sets to produce highly corrected 3D data for retinas over large fields of view. The technology and algorithms used here enabled high transverse resolution imaging of the retina and the choroid at various depths, making the differentiation of morphology visible for the first time within the Sattler’s, Haller’s, and choriocapillaris layers. Visualization of choroidal layers was carried out by using the STOCT angio algorithm. We analyzed the acquired data quantitatively to identify an effective biomarker.
Despite the rapid development of OCT, high-resolution in vivo imaging of human eye with penetration into deeper retinal layers and choroid is still a major challenge due to its sensitivity to coherent noise, such as speckle and crosstalk. To address that, we have developed a technique termed Spatio-Temporal Optical Coherence Tomography (STOC-T) that uses light with controlled spatial and temporal coherence to obtain high-contrasted coronal projection images of the choroid at various depths including that of choriocapillaris. It can also detect blood flow and reveal vascular networks in various chorioretinal layers that are otherwise invisible to OCT.
We present the in-vivo imaging of the global mouse brain ischemia using Bessel beam optical coherence microscopy. This method allows to monitor changes in brain structure with extra control of blood flow during the process of artery occlusion. The results show the capability and sensitivity of OCM system with Bessel beam to analyze brain plasticity after severe injury within a period of 8 days.
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