Ms. Myriam Diaz Martinez Profile

Myriam Diaz Martinez

at Vanderbilt Univ Medical Ctr

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Proceedings Article | 17 March 2015 Paper

Automated pipeline to analyze non-contact infrared images of the paraventricular nucleus specific leptin receptor knock-out mouse model

Myriam Diaz Martinez, Masoud Ghamari-Langroudi, Aliya Gifford, Roger Cone, E. Welch

Proceedings Volume 9417, 94172I (2015) https://doi.org/10.1117/12.2082102

KEYWORDS: Receptors, Thermography, Infrared imaging, Infrared radiation, Neurons, Thermometry, Body temperature, MATLAB, Image analysis, Mouse models

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Evidence of leptin resistance is indicated by elevated leptin levels together with other hallmarks of obesity such as a defect in energy homeostasis.¹ As obesity is an increasing epidemic in the US, the investigation of mechanisms by which leptin resistance has a pathophysiological impact on energy is an intensive field of research.² However, the manner in which leptin resistance contributes to the dysregulation of energy, specifically thermoregulation,³is not known. The aim of this study was to investigate whether the leptin receptor expressed in paraventricular nucleus (PVN) neurons plays a role in thermoregulation at different temperatures. Non-contact infrared (NCIR) thermometry was employed to measure surface body temperature (SBT) of nonanesthetized mice with a specific deletion of the leptin receptor in the PVN after exposure to room (25 °C) and cold (4 °C) temperature. Dorsal side infrared images of wild type (LepRwtwt/sim1-Cre), heterozygous (LepRfloxwt/sim1-Cre) and knock-out (LepRfloxflox/sim1-Cre) mice were collected. Images were input to an automated post-processing pipeline developed in MATLAB to calculate average and maximum SBTs. Linear regression was used to evaluate the relationship between sex, cold exposure and leptin genotype with SBT measurements. Findings indicate that average SBT has a negative relationship to the LepRfloxflox/sim1-Cre genotype, the female sex and cold exposure. However, max SBT is affected by the LepRfloxflox/sim1-Cre genotype and the female sex. In conclusion this data suggests that leptin within the PVN may have a neuroendocrine role in thermoregulation and that NCIR thermometry combined with an automated imaging-processing pipeline is a promising approach to determine SBT in non-anesthetized mice.

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