We report on a flexible multiphoton imaging system, suitable for simultaneous and efficient excitation of red (DsRed), yellow (YFP), green (GFP) and blue (DAPI) fluorophores. We used a simple, compact laser system, consisting of a 1 μm high energy diode-pumped oscillator and a tunable wavelength extension using a photonics crystal fibre. The combination of a near IR excitation wavelength, high energy per pulse for efficient three photon excitation and spectral extension for GFP excitation allows for high flexibility.We present experimental results of simultaneous and efficient
imaging fluorophores couples from the UV to the red (DAPI-RFP, GFP-RFP).
The fiber based generation of nearly transform-limited 10-ps pulses with 200 kW peak power (97 W average power) based on SPM-induced spectral compression is reported. Efficient second harmonic generation applying this source is also discussed.
We have applied a new, 1030 nm wavelength, infrared diode-pumped femtosecond laser source to multiphoton
microscopy, and present comparative results on the efficiency of fluorescence generation versus wavelength for several
fluorophores. It is shown that an emission wavelength of 1030 nm is optimal both for GFP and DsRed excitation.
We report on a novel laser source, emitting high energy (20 nanoJoule) femtosecond pulses, in a broad spectrum (250 nanometers). This source is easily tuned from 950 to 1200 nanometers, without any laser adjustment, and delivers sub-300 femtosecond pulses with a 10 nanometers spectral width.
We report on a novel laser source, emitting high energy (20 nanoJoule) femtosecond pulses, in a broad spectrum (250 nanometers). This source is easily tuned from 950 to 1200 nanometers, without any laser adjustment, and delivers sub-300 femtosecond pulses with a 10 nanometers spectral width.
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