Ball-lens hollow fiber Raman Probe (BHRP) and FTIR spectroscopy were main tools in this study. Thus, both of
equipments detected the alteration of antisymmetric and symmetric P=O stretching vibration within our mice colorectal
tumor models. Some differences of spectra due to randomly the edge of each BHRP and FTIR attached the surface of
tumor during measurements. Meanwhile, the application of FTIR potentially differentiates the grade levels of non-clinic
samples colorectal tumor models at four different grades (normal, grade 1, grade 2 and grade 3). Detailed investigations
were assignable to wave numbers that publicized to represent biochemical alteration. The whole of investigated spectra
in the fingerprint region revealed some different peaks and shoulders, most of which were assignable to wave numbers
that exposed to represent biochemical alteration within the tissue. Differences in peak heights and peak ratio indicated
differences in biochemical composition of cancer from different grade level. However, all collected colorectal tumor
model at different peak was distinguishable, where antisymmetric and symmetric P=O stretching vibration was imaged
and mapped clearly by both equipments. Therefore, BHRP were comfortable for in vivo studies. Meanwhile FTIR
spectral analysis in combination with calibration curve might be used to distinguish cancer grade within colorectal tumor
model tissue for ex vivo study.
In order to estimate the best concentrations of two photosensitizers and the dos of laser each lights for the anti-tumor effect of these photochemical combined treaments. We examined of anti-tumor effect of the photocheical treatment by optical parametric oscillator (OPO) laser lights with two photosensitizers administrations for the transplanted tumor of a cultivated murine leukemia (L5 178Y) cells to Balb/c mude mouse because there is an advantageousness of tunable wavelengths in the OPO laser. Different two photosensitizers of a 2.5ig/gbw pheophorbide-a derivative (PH-1126) and 1mM 5-aminolevulinic acid (5-ALA) as a protoporphyrin IX (Pp-IX) precursor were used when both sensitizers were administrated together. The tumor sizes, the survival fraction and the tumor histological changes of the nude mice were observed after the treatments. From those results, it was found that (1) it was observed the anti-tumor effect by PH-1126 also in the case of the single irrdiation at even 630nm of 5-ALA absorption peak, (2) the combined irradiations at 630nm and 6SOmn for the double administrations were obtained the best anti-tumor effect of the deepened necrosis and pyknosis or disappear-changes of nuclear of the tumor cells about 1 -1.5cm at the depth 1 weak after the laser treatments comparing that of the single treatment for the single photosensitizer. Key words:OPO-laser, two different wavelength irradiations, two different photosensitizers, anti-tumor effect of L5178Y, growth curve, survival fraction
In recent years, the effect of ultrasound (US) in combination with porphyrins such as a gallium-porphyrin complex (ATX-70) has been reported in the treatment of experimental cancers. Before clinical application of this method, further studies are needed. The transplanted squamous cell carcinomas (5CC) on C3H/He mice were irradiated by a tunable optical parametric oscillator (OPO) laser light or ultrasound, in the presence of several different photosensitizers. The tumor size and survival of mice after treatment were observed. Furthermore, we compared antitumor effects of combined irradiation of laser light and ultrasound with those of single laser light or ultrasound, in the presence of ATX-70 or a pheoforbide derivative (PH-1126). Besides PH-i 126 and ATX-70, the following sensitizers were tested: Photofrin II (Pf-II), hematoporphyrin oligomer (HpO). 5-aminolevulinic acid (5-ALA) and aluminum phthalocyanine tetrasuiphonate (AIPcS4). In the results, it was good effects of antitumor by the laser light treatment in the case of PH-1126 and AIPcS4, ATX-70, HpO nsd AIPcS4 produced betterantitumor effeets by irradiation with ultrasound. The antitumor effects of combined use of laser fight and ultrasound were stronger than those of single laser light or ultrasound in both cases of PH-i 126 and ATX-70. Key words: OPO laser light, ultrasound, PH-1126. ATX-70
The photodynamic therapy of cancer has been improved by the developments of laser technology and photosensitizers the last 15 years. The relative yield of active oxygen species generated in the photosensitizing reaction was obtained in vitro by photochemical techniques and ESR measurements with spin trapping reagents in order to find an effective photosensitizer. Cethyltrimethyl ammonium bromide (CTAB) micellar aqueous solutions and human melanoma cultivated (HMF) cell suspensions were used for the in vitro experiments. Photosensitizer was incorporated into the CTAB micelles or HMF cells, and 5,5'-dimethyl-1- pyroline-N-oxide (DMPO) and 2,2,6,6-tetramethyl-4-pyperidone hydrochloride (TMPD) were used for the detection of OH, C (type I reaction) and 1O2* (type II reaction), respectively. After irradiation by laser light or by monochromatic light, the ESR spectra were analyzed. It was found that type I reaction was predominant for the aggregate form of the photosensitizer and type II for the monomer form in CTAB micellar solutions. It was suggested that the predominant type II reaction in HMF cell suspension indicated the monomerization of the photosensitizer by the cells also in the case of hematoporphyrin oligomer (HpO).
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