Dr. Olivier Piot Profile

Dr. Olivier Piot

at Univ de Reims Champagne-Ardenne

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Author

Publications (9)

Proceedings Article | 11 August 2023 Paper

Study of lipid involvement in breast cancer by using vibrational imaging on tissue samples from normal and obese patients

Pooja Mol Girish, Sébastien Legendre, Catherine Muller, Charlotte Vaysse, Emilie Buache, Nicolas Goffin, Olivier Piot

Proceedings Volume 12627, 1262734 (2023) https://doi.org/10.1117/12.2670677

KEYWORDS: Biological samples, Tumors, Adipose tissue, Tissues, Raman spectroscopy, Vibration, Breast cancer, Cancer, Infrared imaging, Statistical analysis

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Breast cancer has become the most diagnosed cancer globally, replacing lung cancer in 2020, with 2.3 million new cases and an estimated death of 685000 women. It is predicted that by the year of 2040 there would be an increase to around 3 million new cases and 1 million deaths worldwide. This calls for techniques for better and faster diagnosis, and in understanding the different biomarkers and the resulting metabolic alterations aiding the development and progression of the tumour. Obesity is associated with metabolic alterations that have shown to increase the risk of cancer and worsen its prognosis. It is associated with dysfunction of adipose tissue that alter the lipid metabolism resulting in excessive accumulation of adipose tissue at sites other than where they are classically found. Tumour cells depend on their microenvironment for nutrients, oxygen and for proliferation. The tumor microenvironment in breast cancer constitutes adipocytes, fibroblasts, endothelial cells, immune cells, and components of the extracellular matrix. The effects of adipocytes on the tumor prognosis are predominant as the breast is composed of abundant fatty tissue. Hence it is important to investigate this effects on molecular levels for understanding the communication between the adipocytes and the tumoral cells which is supporting the proliferation of the cancer. The current diagnostic technique of cancer includes a three step procedure including imaging (such as MRI, Ultrasound imaging), clinical examination and histopathological assessment of biopsy sample if a lesion is suspected to be malignant. However, histopathological assessment observes the morphologic abnormalities in the sample sections and is limited to provide information on the biochemical alterations likely to occur within the tissue even before the morphology is modified. Vibrational spectroscopy has demonstrated its potential to provide diagnostic information. Additionally, vibrational spectroscopy can facilitate the prediction of the biochemical progression for different diseases in a rapid non-destructive manner. Raman spectroscopy is an inelastic scattering process which has incredible potential in biological sample analysis. This technique is capable of rendering information on the vibrational modes of molecules, thus giving access to the biochemical information needed about the sample of interest. Raman spectroscopy is also less time consuming compared to conventional methods of tissue analysis, because the hassle of sample preparation is minimum or not required. The goal of this project is to study the alterations of lipid metabolic pathways in the tumour microenvironment and the impact of obesity in development and progression of breast cancer using vibrational spectroscopy.

Proceedings Article | 27 April 2016 Paper

In vivo diffuse reflectance micro-spectroscopy for correction of Raman depth profiles acquired on skin

Blandine Roig, Anne Koenig, François Perraut, Olivier Piot, Michel Manfait, Jean-Marc Dinten

Proceedings Volume 9887, 988717 (2016) https://doi.org/10.1117/12.2227130

KEYWORDS: Raman spectroscopy, Skin, Scattering, Raman scattering, Tissues, Diffuse reflectance spectroscopy, Confocal microscopy, Light scattering, Skin, Optical properties, Photons, Reflectivity, Absorption, Optical fibers, Monte Carlo methods

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Proceedings Article | 6 March 2015 Paper

Multilayered phantoms with tunable optical properties for a better understanding of light/tissue interactions

Blandine Roig, Anne Koenig, François Perraut, Olivier Piot, Séverine Vignoud, Jonathan Lavaud, Michel Manfait, Jean-Marc Dinten

Proceedings Volume 9325, 93250B (2015) https://doi.org/10.1117/12.2077212

KEYWORDS: Raman spectroscopy, Raman scattering, Scattering, Multilayers, Optical properties, Skin, Absorption, Tissues, X-rays, Titanium dioxide

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Proceedings Article | 5 March 2015 Paper

Biophotonics of skin: method for correction of deep Raman spectra distorted by elastic scattering

Blandine Roig, Anne Koenig, François Perraut, Olivier Piot, Cyril Gobinet, Michel Manfait, Jean-Marc Dinten

Proceedings Volume 9318, 93180L (2015) https://doi.org/10.1117/12.2077216

KEYWORDS: Raman spectroscopy, Raman scattering, Scattering, Confocal microscopy, Optical properties, Skin, Microscopes, Laser scattering, Imaging spectroscopy, Profiling

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SPIE Journal Paper | 19 May 2014 Open Access

Raman spectroscopy: in vivo quick response code of skin physiological status

Raoul Vyumvuhore, Ali Tfayli, Olivier Piot, Maud Le Guillou, Nathalie Guichard, Michel Manfait, Arlette Baillet-Guffroy

JBO, Vol. 19, Issue 11, 111603, (May 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.11.111603

KEYWORDS: Skin, Raman spectroscopy, In vivo imaging, Data modeling, Proteins, Confocal microscopy, Imaging spectroscopy, Biometrics, Biological research, Tissue optics

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SPIE Journal Paper | 1 November 2012 Open Access

Infrared spectral imaging as a novel approach for histopathological recognition in colon cancer diagnosis

Jayakrupakar Nallala, Cyril Gobinet, Marie Danielle Diebold, Valerie Untereiner, Olivier Bouché, Michel Manfait, Ganesh Sockalingum, Olivier Piot

JBO, Vol. 17, Issue 11, 116013, (November 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.11.116013

KEYWORDS: Tissues, Infrared imaging, Tumors, Colon, Imaging spectroscopy, Infrared radiation, Principal component analysis, Proteins, Colorectal cancer, Cancer

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Proceedings Article | 27 February 2006 Paper

In vivo analysis of tissue by Raman microprobe: examination of human skin lesions and esophagus Barrett’s mucosa on an animal model

Ali Tfayli, Olivier Piot, Sylvie Derancourt, Guillaume Cadiot, Marie Diebold, Philippe Bernard, Michel Manfait

Proceedings Volume 6093, 609312 (2006) https://doi.org/10.1117/12.645874

KEYWORDS: Raman spectroscopy, Tissues, Skin, Esophagus, Spectroscopy, In vivo imaging, Skin cancer, Tumor growth modeling, Tissue optics, Optical fibers

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Proceedings Article | 3 May 1999 Paper

Characterization of lipids in wheat grain as probed by microspectrofluorometry

Abdelbasset Saadi, Olivier Piot, Serguei Charonov, Jean-Claude Meunier, Michel Manfait

Proceedings Volume 3602, (1999) https://doi.org/10.1117/12.347526

KEYWORDS: Luminescence, Principal component analysis, Proteins, Imaging spectroscopy, Confocal microscopy, Spectral models, Objectives, Signal detection, Image analysis, Spectroscopy

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Proceedings Article | 9 April 1999 Paper

Confocal Raman microscopic characterization of the molecular species responsible for the grain cohesion of Triticum aestivum wheat: effect of chemical treatment

Olivier Piot, Abdelbasset Saadi, Jean-Claude Autran, Michel Manfait

Proceedings Volume 3608, (1999) https://doi.org/10.1117/12.345407

KEYWORDS: Proteins, Raman spectroscopy, Confocal microscopy, Molecules, Imaging spectroscopy, Luminescence, Spectroscopy, Microscopy, Hydrogen, Visible radiation

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Showing 5 of 9 publications

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