KEYWORDS: Optical coherence tomography, Imaging systems, Cornea, Signal processing, Retina, Cameras, Signal detection, Real time imaging, In vivo imaging, Sensors
A line-field optical coherence tomography (LFOCT) application is implemented for real-time in vivo corneal and retinal imaging. In contrast to other described systems of LFOCT that use single-shot high-speed cameras, we describe the first results utilizing a camera with continuous high-speed data transfer and display. The system is based on a previously published design using a center wavelength of 840nm and a bandwidth of 50nm. The system’s B-frame and en-face display speed reaches up to 5000 frames per second corresponding to 2,500,000 A-lines. A visible light camera is used to detect the interferometric signal to reduce costs and improve optomechanical integration. Balancing the sensitivity vs. acquisition speed allows continuous high data transfer and processing rates and simplifies the implementation as a bedside system. Higher frame rates are important for scan positioning on non-compliant subjects such as infants and children.
KEYWORDS: Signal processing, Optical coherence tomography, Beam diameter, Light sources and illumination, Imaging systems, High speed cameras, Medical device development
A real-time high-speed line-field optical coherence tomography (LFOCT) configuration at 5,000 B-frames or 2,500,000 A-lines per second for clinical applications is described. The development of the LFOCT technology has experienced increasing interest despite the challenge of achieving sufficient sensitivity and resolution due to crosstalk. Publications of research systems usually apply expensive infrared cameras to improve image quality. The costs, complexity, and lack of real-time data transfer of dedicated highspeed IR cameras make it challenging to employ LFOCT for a more comprehensive application range. We demonstrate using a comparatively low-cost, high-speed camera for real-time data transfer and image display in conjunction with a light source at a center wavelength of 840 nm and a bandwidth of 50 nm. Although the camera and available sample power limits the sensitivity (75 dB), we can demonstrate imaging on a human fingertip (in vivo) and the cornea of a rabbit eye. Further development of the system will focus on compensating for the camera's limited sensitivity and improving resolution to target clinical in vivo imaging such as ophthalmic applications.
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