Head and neck cancers are the 16th most prevalent globally, and survival relies on early detection. Widefield autofluorescence (WFAF) shows potential for identifying neoplastic sites but lacks specificity. We investigate multispectral WFAF imaging to identify spectral features from endogenous biomarkers in native and neoplastic oral mucosa, using a DMBA-treated hamster buccal pouch model, with the aim to increase specificity. Spatially resolved spectra revealed variations between nonneoplastic and neoplastic areas. Analyses will compare spectral features in benign, dysplastic, and carcinoma sites from preclinical animal model and human tissues. Refinements in the red/green WFAF approach, by analyzing spectral features, are considered.
A study evaluating the use of multimodal widefield fluorescence imaging with nonlinear optical microscopy for detection of oral neoplasia was carried out using human clinical samples alongside a preclinical model with the goal of optimizing the most relevant endpoint measures to facilitate development and translation. Samples (in vivo buccal mucosa and surgical samples of oral cancer) were imaged by WF imaging using filter selection of red and green spectral windows, multispectral WF imaging to obtain WF spectral characteristics, and multiphoton autofluorescence microscopy. Features between preclinical and human samples were compared. Similarities and relevant endpoints for pursuing further development of a multimodal workflow will be discussed.
The recognized need to develop better clinical approaches for detection of epithelial cancers and potentially malignant lesions than currently used has motivated work in development of noninvasive fluorescence imaging devices. While individual large area imaging and microscopic techniques are promising, recent trends have explored combinations that could merge strengths. The study will discuss a workflow to combine strengths of label-free nonlinear optical microscopy (NLOM) which has shown promise for optical biopsy but is limited in scannable area with widefield autofluorescence microscopy providing large surface area assessment, in studies conducted in both a hamster model for oral neoplasia and inflammation and in surgical oral cancer specimens.
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