The flagellum is vital for eukaryotic cell survival. We propose method utilizing digital holographic microscopy (DHM) advantages, such as high-speed imaging and precise axial localization, to develop a versatile approach for four-dimensional tracking (X, Y, Z, time) of eukaryotic microorganisms' bodies and flagella, addressing challenges faced by existing methods. We reconstructed for the first time the shape of a 200 nm diameter Chrysochromulina simplex flagellum and measured mouse sperm flagella over time, capturing approximately 800 points. Our technique opens new avenues for studying flagella's roles in cellular functions and survival strategies, offering high-speed and precise 3D tracking at the nanoscale.
KEYWORDS: High power lasers, Composites, Polymers, Digital image correlation, Polyurethane, Cameras, Optical coatings, Reflectivity, 3D image processing
In this experimental study we present full methodology for investigation and evaluation of high power laser induced damage and its influence on structural integrity of polymer structural sandwich composites (PSSC) samples covered with various colour paint coatings. The work includes in-situ quantitative monitoring of out-of-plane and in-plane displacements and strains by 3D digital image correlation and temperature fields - by thermovision. The experiments are supported by material reflectance studies and post-mortem, microscopic crater investigations. Conclusions concerning influence of structural core and colour coating on PSSC performance, their structural integrity and laser lethality are presented.
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