A novel LED based light source with a high degree of modularity is resented as a means of illumination in a hyperspectral imaging system, employing multiple LED panes with different wavelengths. We report on first results and experience gained using such illumination in a large area hyperspectral imaging system.
Animal models present a specific set of challenges for hyperspectral imaging. Sample fixation using formalin changes the optical properties of tissues. Since tissues in murine models can be thin and translucent, substrate selection also plays a key role in properly setting up an experimental protocol.
KEYWORDS: Transmittance, Tissues, Monte Carlo methods, Multispectral imaging, Reflectivity, Tissue optics, Bone, Magnetic resonance imaging, Imaging systems
Early detection and treatment of arthritis is essential for a successful outcome of the treatment, but it has proven to be very challenging with existing diagnostic methods. Novel methods based on the optical imaging of the affected joints are becoming an attractive alternative. A non-contact multispectral imaging (MSI) system for imaging of small joints of human hands and feet is being developed. In this work, a numerical simulation of the MSI system is presented. The purpose of the simulation is to determine the optimal design parameters. Inflamed and unaffected human joint models were constructed with a realistic geometry and tissue distributions, based on a MRI scan of a human finger with a spatial resolution of 0.2 mm. The light transport simulation is based on a weighted-photon 3D Monte Carlo method utilizing CUDA GPU acceleration. An uniform illumination of the finger within the 400-1100 nm spectral range was simulated and the photons exiting the joint were recorded using different acceptance angles. From the obtained reflectance and transmittance images the spectral and spatial features most indicative of inflammation were identified. Optimal acceptance angle and spectral bands were determined. This study demonstrates that proper selection of MSI system parameters critically affects ability of a MSI system to discriminate the unaffected and inflamed joints. The presented system design optimization approach could be applied to other pathologies.
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