In this work, we present a novel design and implementation of a lens-free phase imaging system with multi-angle illumination that enhances axial resolution and image quality. The technology, which is based on a common-path shearing interferometer with phase shifting, enables ultra-high sensitivity better than 0.2 nm in optical path difference (OPD), while operating over a wide FoV (>10 mm²) and a large volume (>10 mm³). We show results in several applications, from surface topographies to volumetric structures, including imaging of 10 nm thin transparent topographies and of volumetric laser-written refractive index structures in glass. The high sensitivity and low noise make the proposed technology ideally suited for imaging of low contrast structures on the surface or inside transparent materials, such as defects, impurities, or changes in refractive index.
We propose a disruptive point-of-care (PoC) imaging platform based on lens-free interference phase-contrast imaging for rapid detection of biomarker such as for sepsis and potentially other diseases (e.g. cancer). It enables simultaneous analysis of potentially up to 10,000 functionalized microarray spots with different biomarkers with fast time-to-results (few minutes) and by consuming a small sample volume (~10 μL). The high sensitivity allows direct measurements of the biomarker binding without the use of fluorescent labels (e.g. ELISA) or microbial culture methods. In addition, adhoc plasmonic nano-structuring is utilized to significantly improve the sensitivity for biomarker detection (optical path difference ~Å) to concentration levels relevant for disease diagnosis.
The proposed technology incorporates a portable and low-cost lens-free imaging reader made of consumer electronic components, plasmonic microarrays with distinct functionalization, and user-friendly software based on a novel phaseshifting interferometry method for topography and refractive index analysis. Due to its compactness and cost-efficiency, we foresee a great potential for PoC applications, especially for the rapid detection of infectious diseases or lifethreatening conditions, e.g. sepsis, but also for clinical trials of drugs and food control.
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