Fiber-optic surface plasmon resonance (SPR) sensors have the advantages of high sensitivity and fast response. Traditional single-channel sensors cannot fulfill practical detection needs, while multi-channel fiber-optic SPR sensors can achieve a diversity of analyte determinations. Therefore, developing a multi-channel fiber-optic SPR detection system is significant for field detection. To solve the issues of single-channel detection and lack of reference channels in fiber-optic SPR, we propose a multi-channel fiber-optic SPR real-time monitoring system. This system uses the LabVIEW platform for system design. It can realize the function of multi-channel dynamic monitoring. The experimental results showed that the four independent channel has similar sensitivity. The result confirmed the multi-channel SPR sensors have excellent stability and repeatability. To further validate the reliability of the multi-channel sensors in bio-detection, the different concentrations of ConA solution were tested. The constructed multi-channel SPR sensing system has an excellent linear response in the 0.05~0.2 mg/mL range. The wavelength shift of a single channel could reach 0.58~1.55 nm in the range of 0.05~0.2 mg/ml with a linearity of 0.98, which verified the biochemical detection capability of the system. Furthermore, the ConA solution of the same concentration was used to test sensitivity for different channels. The experiment shows that the four independent channels have similar resonance wavelength shifts (0.772 nm, 0.814 nm, 0.7968 nm, and 0.799 nm, respectively). The proposed multi-channel fiber-optic SPR sensing system has applications in the field of multi-analyte detection.
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