Various techniques have been used for preparation of gelatin nanoparticles, such as coacervation, emulsion/solvent
evaporation, reverse phase preparation, inverse miniemulsion and two step desolvation. Both methods are based on
different mechanisms of nanoparticle formation. The main goal of this study was to systematically compare the
performance of nanoprecipitation and the most widely utilized two step desolvation methods with respect to effect of
gelatin concentration on nanoparticle size and polydispersity index. Particles size was determined by dynamic light
scattering, and the morphology by atomic force microscopy. It was observed that gelatin concentration 20 mg/ml yielded nanoparticles of around 60 nm size by two step desolvation, on the other hand nanoprecipitation produced 210 nm particles with the same gelatin concentration.
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