Ms. Sangita Sridharan Profile

Sangita Sridharan

at The Univ. of Toledo Medical Center

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Proceedings Article | 2 January 2018 Paper

Study of interaction of GNR with glioblastoma cells

Arti Hole, P. Cardoso-Avila, Sangita Sridharan, Aditi Sahu, Jyothi Nair, Harsh Dongre, Jayant Goda, Sharada Sawant, Shilpee Dutt, J. Pichardo-Molina, C. Murali Krishna

Proceedings Volume 10456, 1045623 (2018) https://doi.org/10.1117/12.2282553

KEYWORDS: Nanoparticles, Gold, Nanorods, Raman spectroscopy, Transmission electron microscopy, Tissues, Radiotherapy, Cancer, Tumors, Brain cancer

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Radiation resistance is one of the major causes of recurrence and failure of radiotherapy. Different methods have been used to increase the efficacy of radiation therapy and at the same time restrict the radiation resistivity. From last few years nanoparticles have played a key role in the enhancement of radiosensitization. The densely packed nanoparticles can selectively scatter or absorb the high radiations, which allow better targeting of cellular components within the tumor hence resulting in increased radiation damage to the cancer cells. Glioblastoma multiforme (GBM) is one of the highly radioresistant brain cancer. Current treatment methods are surgical resection followed by concurrent chemo and radiation therapy. In this study we have used in-house engineered gold nano rodes (GNR) and analyzed their effect on U-87MG cell lines. MTT assay was employed to determine the cytotoxic concentration of the nanoparticles. Raman spectroscopy was used to analyze the effect of gold nanoparticles on glioma cells, which was followed by transmission electron microscopic examinations to visualize their cellular penetration. Our data shows that GNR were able to penetrate the cells and induce cytotoxicity at the concentration of 198 μM as determined by MTT assay at 24 post GNP treatment. Additionally, we show that Raman spectroscopy, could classify spectra between untreated and cells treated with nanoparticles. Taken together, this study shows GNR penetration and cytotoxicity in glioma cells thereby providing a rationale to use them in cancer therapeutics. Future studies will be carried out to study the biological activity of the formulation as a radiosensitizer in GBM.

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