Mr. Seonkyung Lee Profile

Seonkyung Lee

at SRI International

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Proceedings Article | 12 September 2003 Paper

Optical property measurements of a novel type of upconverting reporter

Xudong Xiao, Michael Herring, Jeanne Haushalter, Seonkyung Lee, Kostas Kalogerakis, Gregory Faris

Proceedings Volume 4967, (2003) https://doi.org/10.1117/12.479841

KEYWORDS: Neodymium, Upconversion, Ions, Absorption, Biomedical optics, Diagnostics, Lanthanides, Molecules, Optical properties, Optical testing

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We have recently developed a new type of reporter (upconverting chelate) for biomedical diagnostics. For this reporter, the light is absorbed and emitted by a lanthanide ion, rather than an organic molecule, as is the case for a typical fluorescent dye. These materials do not photobleach and have no autofluorescent background. We focus in this paper on neodymium ions complexed with the familiar chelating agents, EDTA, DPA, DTPA and DOTA. We have performed experimental measurements with one- and two-color laser light excitation for different chelate compounds. The samples are excited using two Nd:YAG-pumped dye laser systems that provide laser light near 587 nm and 800 nm. For one-color excitation, the emitted light depends quadratically on the incident laser power, as expected. Three strongly emitting lines are observed, located near 360 nm, 387 nm, and 417 nm. We observed more efficient upconversion in EDTA although the DPA chelates show comparable ground state absorbance. We have studied the influence of temporal delay between the two laser pulses and obtained the decay lifetime of the first intermediate state in the various chelated compounds.

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