In this work, we demonstrate a method that can focus on multiple single guidestar through scattering medium with a spatial light modulator (SLM) and a bucket detector using two-photon fluorescence signals. A gradient descent based multiplexed phase retrieval algorithm is used to non-invasively reconstruct the transmission matrix between the guidestars and the SLM, without any assumptions on the memory effect range. Conversely, if we consider the memory effect, we can reconstruct the image of the sample.
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