KEYWORDS: Luminescence, In vivo imaging, Collagen, Inflammation, Imaging systems, Visualization, Animal model studies, Near infrared, Pathology, Cartilage
An in vivo near-infrared fluorescence (NIRF) imaging technique is described for therapy monitoring of ankle joints affected by collagen-induced arthritis, a model of human rheumatoid arthritis. Arthritis was induced in rats by intradermal injections of collagen and Freund’s incomplete adjuvant. For in vivo imaging, the nonspecific NIR dye tetrasulfocyanine (TSC) was used. Prior to and after treatment with a nonsteroidal anti-inflammatory drug, meloxicam, or analgesic drug, tramadol hydrochloride (which served as no-therapy control), normalized fluorescence intensities of each ankle joint were measured. Additionally, each ankle joint was characterized by clinical arthritis scoring and histopathology. Over a 3-week treatment period, a significant difference in disease progression between animals treated with meloxicam and tramadol hydrochloride was detected. A statistically significant improvement in ankle joint pathology from high- or moderate-grade to moderate- or low-grade upon meloxicam therapy, as determined by clinical evaluation, translated into a significant decrease in fluorescence intensity. In contrast, all arthritic joints of the no-therapy control group deteriorated to high-grade arthritis with high-fluorescence intensities in NIRF imaging.
KEYWORDS: Luminescence, Scanners, Imaging systems, Magnetic resonance imaging, In vivo imaging, Scattering, Near infrared, Spatial resolution, Animal model studies, Head
We have developed a flying-spot scanner for fluorescence imaging of rheumatoid arthritis in the near infrared (NIR)
spectral range following intravenous administration of contrast agents. The new imaging system has been characterized
with respect to linearity, dynamic range and spatial resolution with the help of fluorescent phantoms. In vivo experiments
were performed on an animal model of rheumatoid arthritis. Finally, NIR-fluorescence images of early stages of joint
inflammation have been compared with findings from contrast enhanced MR imaging and histology.
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