Dr. Spencer L. Shorte Profile

Dr. Spencer L. Shorte

Director at Institut Pasteur

SPIE Involvement:

Author

Publications (14)

Proceedings Article | 12 March 2024 Paper

ZOLA 3D, single molecule microscope technological transfer from research lab to ISO 9001 core facility

Yumi Gosselin, Mickael Lelek, Audrey Salles, Christophe Zimmer, Spencer Shorte, Elric Esposito

Proceedings Volume 12849, 1284908 (2024) https://doi.org/10.1117/12.3002293

KEYWORDS: Point spread functions, 3D image processing, Microscopes, Biological imaging, 3D modeling, Molecules, Biological samples, Imaging systems, Education and training

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Proceedings Article | 10 March 2015 Paper

Spatio-angular light control in microscopes using micro mirror arrays

F. Rückerl, S. Bellow, D. Berndt, J-Y. Tinevez, Jörg Heber, M. Wagner, S. Shorte

Proceedings Volume 9305, 93052Y (2015) https://doi.org/10.1117/12.2079466

KEYWORDS: Micromirrors, Mirrors, Optogenetics, Spatial light modulators, Microscopy, Neodymium, Microscopes, Ions, Control systems, Objectives

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Proceedings Article | 9 March 2015 Paper

A new method to achieve tens of nm axial super-localization based on conical diffraction PSF shaping

Clément Fallet, Maxime Dubois, Jean-Yves Tinevez, Stephane Oddos, Julien Caron, Roger Persson, Spencer Shorte, Gabriel Sirat

Proceedings Volume 9331, 93310Q (2015) https://doi.org/10.1117/12.2077712

KEYWORDS: Point spread functions, Crystals, Polarizers, Polarization, Cameras, Error analysis, Diffraction, Laser crystals, Photon polarization, Molecules

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Proceedings Article | 27 February 2015 Paper

Spatially resolved contrast measurement of diffractive micromirror arrays

Cornelius Sicker, Jörg Heber, Dirk Berndt, Florian Rückerl, Jean-Yves Tinevez, Spencer Shorte, Michael Wagner, Harald Schenk

Proceedings Volume 9375, 93750D (2015) https://doi.org/10.1117/12.2076921

KEYWORDS: Micromirrors, Diffraction, Scatter measurement, Mirrors, Light scattering, Cameras, Sensors, Interferometry, CCD cameras, Stray light

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Proceedings Article | 28 August 2014 Paper

Conical diffraction as a versatile building block to implement new imaging modalities for superresolution in fluorescence microscopy

Clément Fallet, Julien Caron, Stephane Oddos, Jean-Yves Tinevez, Lionel Moisan, Gabriel Sirat, Philippe Braitbart, Spencer Shorte

Proceedings Volume 9169, 916905 (2014) https://doi.org/10.1117/12.2061059

KEYWORDS: Super resolution, Crystals, Microscopy, Polarization, Diffraction, Confocal microscopy, Laser crystals, Molecules, Luminescence, Cameras

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Proceedings Article | 2 May 2014 Paper

Photoactivation and optogenetics with micro mirror enhanced illumination

F. Rückerl, D. Berndt, J. Heber, S. Shorte

Proceedings Volume 9130, 913017 (2014) https://doi.org/10.1117/12.2052556

KEYWORDS: Mirrors, Micromirrors, Optogenetics, Neodymium, Ultraviolet radiation, Objectives, Spatial light modulators, Beam steering, Microscopes, Microscopy

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SPIE Journal Paper | 5 March 2014 Open Access

Real-time label-free detection of dividing cells by means of lensfree video-microscopy

Srikanth Kesavan, Fabrice Navarro Y Garcia, Mathilde Menneteau, Frédérique Mittler, Brigitte David-Watine, Nelly Dubrulle, Spencer Shorte, Bernard Chalmond, Jean-Marc Dinten, Cédric Allier

JBO, Vol. 19, Issue 03, 036004, (March 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.3.036004

KEYWORDS: Holography, Video, Pattern recognition, Sensors, Image acquisition, 3D image reconstruction, Oncology, Optical sensors, Light emitting diodes, Fetus

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Proceedings Article | 4 March 2014 Paper

Video lensfree microscopy of 2D and 3D culture of cells

C. Allier, S. Vinjimore Kesavan, J.-G. Coutard, O. Cioni, F. Momey, F. Navarro, M. Menneteau, B. Chalmond, P. Obeid, V. Haguet, B. David-Watine, N. Dubrulle, S. Shorte, B. van der Sanden, C. Di Natale, L. Hamard, D. Wion, M. Dolega, N. Picollet-D'hahan, X. Gidrol, J.-M. Dinten

Proceedings Volume 8947, 89471H (2014) https://doi.org/10.1117/12.2038098

KEYWORDS: Video microscopy, Video, Microscopy, Holography, Tissues, Microscopes, Biology, Image sensors, 3D modeling, Image segmentation

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Proceedings Article | 8 March 2013 Paper

Micro mirror arrays as high-resolution spatial light modulators for photoactivation and optogenetics

F. Rückerl, Martin Kielhorn, J-Y. Tinevez, J. Heber, R. Heintzmann, S. Shorte

Proceedings Volume 8586, 85860U (2013) https://doi.org/10.1117/12.2010408

KEYWORDS: Mirrors, Microscopy, Micromirrors, Optogenetics, Microscopes, Spatial light modulators, Liquid crystal on silicon, Luminescence, Confocal microscopy, Image quality

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Proceedings Article | 23 February 2011 Paper

Validation of method for enhanced production of red-shifted bioluminescent photons in vivo

Joe Dragavon, Samantha Blazquez, Kelly Rogers, Chelsea Samson, Régis Tournebize, Spencer Shorte

Proceedings Volume 7902, 790210 (2011) https://doi.org/10.1117/12.875416

KEYWORDS: Photons, Luminescence, Bioluminescence, Bacteria, In vivo imaging, Tissues, Absorption, Picosecond phenomena, Absorbance, In vitro testing

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Proceedings Article | 12 February 2011 Paper

Fluorescence lifetime imaging to quantify sub-cellular oxygen measurements in live macrophage during bacterial invasion

Joe Dragavon, Megdouda Amiri, Benoit Marteyn, Philipe Sansonetti, Spencer Shorte

Proceedings Volume 7910, 791019 (2011) https://doi.org/10.1117/12.875430

KEYWORDS: Oxygen, Fluorescence lifetime imaging, Luminescence, Bacteria, Pathogens, Electrodes, Carbon dioxide, Cell death, In vitro testing, Optical filters

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SPIE Journal Paper | 1 May 2008 Open Access

Electron-multiplying charge-coupled detector-based bioluminescence recording of single-cell Ca2+

Kelly Rogers, Jean-Rene Martin, Olivier Renaud, Eric Karplus, Marie-Anne Nicola, Marie Nguyen, Sandrine Picaud, Spencer Shorte, Philippe Brulet

JBO, Vol. 13, Issue 03, 031211, (May 2008) https://doi.org/10.1117/12.10.1117/1.2937236

KEYWORDS: Calcium, Bioluminescence, Electron multiplying charge coupled devices, Sensors, Green fluorescent protein, Microscopes, Signal detection, Luminescence, Acquisition tracking and pointing, Video

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Proceedings Article | 19 February 2007 Paper

A system and methodology for high-content visual screening of individual intact living cells in suspension

Olivier Renaud, Rainer Heintzmann, Asier Sáez-Cirión, Thomas Schnelle, Torsten Mueller, Spencer Shorte

Proceedings Volume 6441, 64410Q (2007) https://doi.org/10.1117/12.699000

KEYWORDS: Visualization, Confocal microscopy, Microscopes, 3D image processing, Stereoscopy, Dielectrics, 3D acquisition, Electrodes, Imaging systems, Fluorescence tomography

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Proceedings Article | 23 February 2006 Paper

Three-dimensional FRET microscopy

Adam Hoppe, Joel Swanson, Spencer Shorte

Proceedings Volume 6089, 608904 (2006) https://doi.org/10.1117/12.654819

KEYWORDS: Fluorescence resonance energy transfer, Deconvolution, Microscopes, Microscopy, Signal detection, Proteins, 3D image processing, Data modeling, Luminescence, Confocal microscopy

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Showing 5 of 14 publications

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