The first continuous flow micro PCR introduced in 1998 has attracted considerable attention for the past several years
because of its ability to amplify DNA at much faster rate than the conventional PCR and micro chamber PCR method.
The amplification is obtained by moving the sample through 3 different fixed temperature zones. In this paper, the
thermal behavior of a continuous flow PCR chip is studied using commercially available finite element software. We
study the temperature uniformity and temperature gradient on the chip's top surface, the cover plate and the interface of
the two layers. The material for the chip body and cover plate is glass. The duration for the PCR chip to achieve
equilibrium temperature is also studied.
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