A port-wine stain is a congenital vascular malformation, which may worsen if left untreated.
Selective photothermolysis with Pulse-dye lasers is a well-established treatment for PWS. However, outcomes are often unpredictable due to tissue heterogeneity. This study characterizes the three-dimensional architecture of ex vivo human skin from control and PWS regions of five patients before and after laser treatment using optical coherence tomography (OCT), two-photon excitation fluorescence microscopy (TPEM), and second harmonic generation (SHG). Results suggest that combining the high penetration depth of OCT with the high resolution of multiphoton imaging could provide a means for real-time guidance of selective phototherapy.
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