The aim of the study is investigation of morphological features of the testicles of rat newborns with different models of intrauterine hypoxia. Fifteen white outbred female rats aged from 4 to 10 months with a weight of 200 ± 30 g were used. Laboratory animals were divided into 3 experimental groups, 5 rats in each. The first group was subjected to a hypoxic hypoxia during pregnancy (21 days). The hypoxia was simulated in accordance with the method of N.N. Karkishchenko (2010). The second group was subjected to a hemic hypoxia during the second and third weeks of pregnancy, in accordance with the methodology of L.M. Sosedova (2012). The third (control) group was not exposed to any treatment during the pregnancy. After delivery, the newborn rat pups were subjected to the measurements of oxygenation degree using the methods of reflectance spectroscopy, then the animals were withdrawn from the experiment; and a morphological study of the testicle tissues was performed. Histological examination of the tissues of the testicles of newborn rats showed signs of impaired blood circulation, dystrophic and necrobiotic changes in the parenchyma.
The aim of work was to study the characteristics of uteroplacental blood flow in pregnant rats, tissue oxygenation and the morphological features of the main internal organs (heart, liver, kidneys) of newborns during chronic intrauterine hypoxia. The rats (8 animals) were divided into 2 experimental groups. The first (hypoxia) group was subjected to hypoxia throughout pregnancy (21 days). The second (control) group was not exposed to any effect throughout the pregnancy. On the 20th day of gestation in both groups, a Doppler ultrasound study of uterine-fetal blood flow and measurement of the placenta was performed. Newborn rat pups of the first day of life were subjected to oxygenation degree measurement. After delivery, the number of rats in the litter was counted and their body weight was measured. After the measurements the laboratory animals were withdrawn from the experiments and morphological study of their internal organs was performed. The studies have shown a clear relationship between the characteristics of uterine blood flow and the presence or absence of hypoxia. In chronic hypoxia, there were low rates of arterial blood flow, an increase in the peripheral resistance of uterine vessels. All this indicates the defective formation of the placenta and impaired blood flow with the development of placental insufficiency. It was also found that hypoxia in the antenatal period adversely affects the number and somatometric indicators of newborn rats in the offspring. Histological examination of the tissues of the heart, liver and kidney of newborn rat pups showed signs of impaired blood circulation, dystrophic and necrobiotic changes in the parenchyma.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.