Influence of illuminating beam Gauss parameter to Biological Compact Disc Sensor (BioCD) is presented
in this paper. BioCD is a sensitive Interferometry detection platform to detect bimolecular immobilized on
the surface of a spinning disk. Interferometry has the advantage of higher photon fluxes than conventional
fluorescence detection and consequently shorter detection time. It allows high speed detection of optical
path length changes down to sub-nanometer scales with high repeatability. The influences of illuminating
beam Gauss parameter to detection sensitivity were simulated. Simulation result showed that Gauss
parameter increase will reduce detection sensitivity. But if there is a pinhole filter in optical system Gauss
parameter influence could be ignored. This also will be help to Suppression influence of lens aberration, so
improve BioCD detection sensitivity.
The theoretical model and calibration experiment of biological compact disc sensor (BioCD) is presented. BioCD is a
new kind of label-free optical biosensor based on Interferometry to detect immobilized biomolecules with high speed,
high sensitivity and high-throughput. Output signal theoretical expressions were deduced. The influences of illuminating
beam Gauss parameter and lens aberration to detection sensitivity were simulated. Simulation result showed that Gauss
parameter as well as aberration parameter increase will reduce detection sensitivity. Then BioCD experimental platform
was setup, sensor arrays for calibration was fabricated using reactive ion etching technology on silicon substrate.
Experimental results indicated that detection sensitivity can achieve 15mV/nm.
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