Snapshot Image Mapping Spectrometer (IMS) allows to obtain 3D (x,y,lamda) datacubes instantaneously in a snapshot mode. The multifaceted mapping mirror is a critical component in the IMS for creating void spaces between image lines for spectral information. Here we present a completely new fabrication technique for mapping mirrors based on lithographic Two-Photon Polymerization (2PP). A grayscale 16-bit pixelized mapping mirror was designed in MATLAB, which allows to adopt 1-micron slicing distance and 0.2-micron hatching distance to keep low surface roughness while reducing printing time. The instability issue of the surface structure can be solved by adding two bases. Many other parameters (attenuation, develop time) used in 2PP printing were also optimized to provide the best surface quality. The new fabrication method decreases the facet shadowing (smaller height discrepancies), provides uniform image intensity, and enables easy reproducibility.
Image mapping spectrometer (IMS) allows to obtain 3D (x,y,λ) datacubes instantaneously in a snapshot mode. It has a wide range of applications, including cell signaling, cancer diagnostics, and retinal imaging. The key component, multifaceted mapping mirrors, were fabricated by diamond machining, which have issues causing the variant intensity of facets and limited spatial samplings. Here we present an entirely new fabrication technique using lithographic Two-Photon Polymerization (2PP). A pixelized mapping mirror with an aluminum coating was designed and fabricated to overcome the challenges brought by prior mapping mirrors. A prototype IMS was set up on the bench to show preliminary fluorescence hyperspectral images.
The molecular mechanisms that define cellular functions can be elucidated by live-cell hyperspectral fluorescence microscopy, which permits simultaneous measurements of multiple dynamic processes with excellent signal-to-noise ratios. We have developed a novel five-dimensional (x,y,z,t,λ) imaging system by combining dual-view Selective-Plane Illumination Microscopy (diSPIM) for isotropic diffraction-limited imaging and image mapping spectroscopy (IMS) for single snapshot hyperspectral imaging of multiple fluorophores. We will demonstrate the properties of this instrument and show its application to imaging islet of Langerhans, a micro-organ that contributes to blood glucose homeostasis through the regulated secretion of glucagon (from α-cells) and insulin (from β-cells).
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