A new nano-structured SPR sensor was devised to improve its sensitivity. Nano-scaled silica particles were used as the
template to fabricate nano-structure. The surface of the silica particles was modified with thiol group and a single layer
of the modified silica particles was attached on the gold or silver thin film using Langmuir-Blodgett (LB) method.
Thereafter, gold or silver was coated on the template by an e-beam evaporator. Finally, the nano-structured surface with
basin-like shape was obtained after removing the silica particles by sonication. Applying the new developed SPR sensor
to a model food of alcoholic beverage, the sensitivities for the gold and silver nano-structured sensors, respectively, had
95% and 126% higher than the conventional one.
Nanotechnology applications for food safety and biosecurity, especially development of nanoscale sensors for foodborne
pathogen measurement are emerging. A novel bio-functional nanosensor for Salmonella detection was developed using
hetero-nanorods. The silica nanorods were fabricated by glancing angle deposition method and the gold was sputtered
onto the silica nanorods. Alexa488-succinimide dye was immobilized onto the annealed Si nanorods via the attachment
between dye ester and primary amine group supplied by the
3-Aminopropyltriethoxysilane. The anti-Salmonella was
conjugated to gold via Dithiobis[succinimidylpropionate]
self-assembly monolayer. Due to the high aspect ratio nature
of the Si nanorods, hundreds or thousands of dye molecules attached to the Si nanorods produced enhanced fluorescence
signal. These biologically functionalized nanorods can be used to detect Salmonella with fluorescent microscopic
imaging. This new nanoscale biosensor will be able to detect other foodborne pathogenic bacteria for food safety and
security applications.
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