Prof. Yonghong Shao Profile

Prof. Yonghong Shao

at Shenzhen Univ

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Author

Publications (22)

Proceedings Article | 14 February 2020 Paper

Study of the sarcomeric addition process in a tissue-like cell construct under mechanical overload via TPEF-SHG imaging system

Ailin Wei, Zhonghai Wang, Zongming Yang, Shenghao Tan, Tong Ye, Yonghong Shao, Thomas Borg, Bruce Gao

Proceedings Volume 11244, 112442E (2020) https://doi.org/10.1117/12.2546280

KEYWORDS: Two photon excitation microscopy, Imaging systems, Heart, Beam splitters, Second-harmonic generation, Tissues, Microscopes

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Proceedings Article | 19 April 2017 Presentation

Polarization-resolved SHG microscopy in cardiac hypertrophy study (Conference Presentation)

Zhonghai Wang, Cai Yuan, Yonghong Shao, Amy Bradshaw, Thomas Borg, Bruce Gao

Proceedings Volume 10042, 100420M (2017) https://doi.org/10.1117/12.2253161

KEYWORDS: Medical research, Heart, Tissues, Microscopy, Second-harmonic generation, Polarization, Harmonic generation, Visualization, Molecular biology, Diagnostics

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Cardiac hypertrophy, a process initiated by mechanical alterations, is hypothesized to cause long-term molecular-level alteration in the sarcomere lattice, which is the main force-generating component in the heart muscle. This molecular-level alteration is beyond the resolving capacity of common light microscopy. Second harmonic generation (SHG) microscopy has unique capability for visualizing ordered molecular structures in biological tissues without labeling. Combined with polarization imaging technique, SHG microscopy is able to extract structural details of myosin at the molecular level so as to reveal molecular-level alterations that occur during hypertrophy. The myosin filaments are believed to possess C6 symmetry; thus, the nonlinear polarization response relationship between generated second harmonic light I^2ωand incident fundamental light I^ω is determined by nonlinear coefficients, χ_15, χ_31 and χ_33. χ_31/χ_15 is believed to be an indicator of the molecular symmetry of myosin filament, whileχ_33/χ_15represents the intramyosin orientation angle of the double helix. By changing the polarization of the incident light and evaluating the corresponding SHG signals, the molecular structure of the myosin, reflected by the χ coefficients, can be revealed. With this method, we studied the structural properties of heart tissues in different conditions, including those in normal, physiologically hypertrophic (heart tissue from postpartum female rats), and pathologically hypertrophic (heart tissue from transverse-aorta constricted rats) conditions. We found that ratios of χ_31/χ_15 showed no significant difference between heart tissues from different conditions; their values were all close to 1, which demonstrated that Kleinman symmetry held for all conditions. Ratios of χ_33/χ_15 from physiologically or pathologically hypertrophic heart tissues were raised and showed significant difference from those from normal heart tissues, which indicated that the intramyosin orientation angle of the double helix was altered when heart tissues hypertrophied. Polarization-resolved SHG microscopy permitted us to study heart tissues at the molecular level and may serve as a diagnostic tool for cardiac hypertrophy.

SPIE Journal Paper | 9 December 2016 Open Access

Fast spectral surface plasmon resonance imaging sensor for real-time high-throughput detection of biomolecular interactions

Kaiqiang Chen, Youjun Zeng, Lei Wang, Dayong Gu, Jianan He, Shu-Yuen Wu, Ho-Pui Ho, Xuejin Li, Junle Qu, Bruce Zhi Gao, Yonghong Shao

JBO, Vol. 21, Issue 12, 127003, (December 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.12.127003

KEYWORDS: Sensors, Surface plasmons, Optical sensors, Feedback loops, Imaging systems, Biosensors, Tunable filters, Spectral resolution, Time metrology, Charge-coupled devices

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Proceedings Article | 28 February 2014 Paper

Pulse splitter-based nonlinear microscopy for live-cardiomyocyte imaging

Zhonghai Wang, Wan Qin, Yonghong Shao, Siyu Ma, Thomas Borg, Bruce Gao

Proceedings Volume 8948, 89482X (2014) https://doi.org/10.1117/12.2041845

KEYWORDS: Second-harmonic generation, Imaging systems, Beam splitters, Microscopy, Microscopes, Mirrors, Objectives, Laser safety, Cell mechanics, Electronic filtering

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Proceedings Article | 28 September 2013 Paper

1D-scanning addressable multiregional multifocal multiphoton microscopy

Wan Qin, Yonghong Shao, Honghai Liu, Xiang Peng, Hanben Niu, Bruce Gao

Proceedings Volume 8845, 884517 (2013) https://doi.org/10.1117/12.2024325

KEYWORDS: Spatial light modulators, Multiphoton microscopy, Imaging systems, Cameras, Beam splitters, Computer generated holography, Image resolution, Confocal microscopy, Electron multiplying charge coupled devices, Microscopes

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Proceedings Article | 26 February 2013 Paper

Disassembly of myofibrils in adult cardiomyocytes during dedifferentiation

Honghai Liu, Wan Qin, Yonghong Shao, Zhonghai Wang, Huaxiao Yang, Raymond Runyan, Thomas Borg, Bruce Gao

Proceedings Volume 8587, 85871W (2013) https://doi.org/10.1117/12.2005156

KEYWORDS: Imaging systems, Second-harmonic generation, Tissues, Heart, In vitro testing, Multichannel imaging systems, Signal detection, Luminescence, Carbon dioxide, Microscopes

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SPIE Journal Paper | 1 February 2013 Open Access

Micro-Raman spectroscopy study of cancerous and normal nasopharyngeal tissues

Yongzeng Li, Jianji Pan, Guannan Chen, Chao Li, Shaojun Lin, Yonghong Shao, Shangyuan Feng, Zufang Huang, Shusen Xie, Haishan Zeng, Rong Chen

JBO, Vol. 18, Issue 02, 027003, (February 2013) https://doi.org/10.1117/12.10.1117/1.JBO.18.2.027003

KEYWORDS: Tissues, Raman spectroscopy, Diagnostics, Cancer, Micro raman spectroscopy, Principal component analysis, Proteins, Tumors, Lithium, Statistical analysis

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Proceedings Article | 11 December 2012 Paper

Study of ABO blood types by combining membrane electrophoresis with surface-enhanced Raman spectroscopy

Jing Wang, Juqiang Lin, Zufang Huang, Liqing Sun, Yonghong Shao, Peng Lu, Wei Shi, Jinyong Lin, Rong Chen

Proceedings Volume 8553, 855323 (2012) https://doi.org/10.1117/12.1000238

KEYWORDS: Blood, Surface enhanced Raman spectroscopy, Raman spectroscopy, Plasma, Silver, Principal component analysis, Nanoparticles, Genetics, Proteins, Raman scattering

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Proceedings Article | 11 December 2012 Paper

Surface-enhanced Raman spectroscopy study of radix astragali based on soxhlet extractor

Peng Lu, Juqiang Lin, Nengrong Liu, Yonghong Shao, Jing Wang, Wei Shi, Jinyong Lin, Rong Chen

Proceedings Volume 8553, 855325 (2012) https://doi.org/10.1117/12.1000240

KEYWORDS: Raman spectroscopy, Surface enhanced Raman spectroscopy, Silver, Solids, Nanoparticles, Medicine, Signal detection, Solid state physics, Biomedical optics, Cardiovascular system

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SPIE Journal Paper | 3 April 2012 Open Access

Addressable multiregional and multifocal multiphoton microscopy based on a spatial light modulator

Yonghong Shao, Junle Qu, Xiang Peng, Hanben Niu, Wan Qin, Honghai Liu, Bruce Gao

JBO, Vol. 17, Issue 03, 030505, (April 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.3.030505

KEYWORDS: Spatial light modulators, Multiphoton microscopy, Scanners, Microscopes, Image resolution, Confocal microscopy, Beam splitters, Mirrors, Diffractive optical elements, Microlens

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Proceedings Article | 23 February 2012 Paper

Live cardiomyocyte imaging via hybrid TPEF-SHG microscopy

Honghai Liu, Wan Qin, Yonghong Shao, Qiuying Liu, Zhen Ma, Thomas Borg, Bruce Gao

Proceedings Volume 8225, 822524 (2012) https://doi.org/10.1117/12.908381

KEYWORDS: Second-harmonic generation, Imaging systems, Proteins, Microscopy, Luminescence, Harmonic generation, Femtosecond phenomena, Assembly equipment, Tissues, Wave propagation

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Proceedings Article | 23 February 2012 Paper

Multifocus nonlinear optical microscopy based on SLM

Wan Qin, Yonghong Shao, Honghai Liu, Junle Qu, Xiang Peng, Hanben Niu, Bruce Gao

Proceedings Volume 8226, 82262R (2012) https://doi.org/10.1117/12.909558

KEYWORDS: Spatial light modulators, Image resolution, Microscopes, Imaging systems, Cameras, Multiphoton microscopy, Beam splitters, Computer generated holography, Spatial resolution, Electron multiplying charge coupled devices

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SPIE Journal Paper | 1 December 2011 Open Access

Myofibrillogenesis in live neonatal cardiomyocytes observed with hybrid two-photon excitation fluorescence-second harmonic generation microscopy

Honghai Liu, Wan Qin, Zhen Ma, Bruce Gao, Yonghong Shao, Tong Ye, Thomas Borg

JBO, Vol. 16, Issue 12, 126012, (December 2011) https://doi.org/10.1117/12.10.1117/1.3662457

KEYWORDS: Imaging systems, Second-harmonic generation, Harmonic generation, Microscopes, Microscopy, Luminescence, Green fluorescent protein, Beam splitters, Objectives, Signal detection

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Proceedings Article | 3 March 2011 Paper

3D myofibril imaging in live cardiomyocytes via hybrid SHG-TPEF microscopy

Yonghong Shao, Honghai Liu, Tong Ye, Tom Borg, Junle Qu, Xiang Peng, Hanben Niu, Bruce Gao

Proceedings Volume 7903, 79030F (2011) https://doi.org/10.1117/12.875607

KEYWORDS: Second-harmonic generation, Heart, 3D image processing, Imaging systems, Microscopes, Polarization, Scanners, Stereoscopy, Microscopy, Luminescence

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Proceedings Article | 21 February 2008 Paper

A fluorescence sectioning endoscopy using dynamic speckle illumination

Jun Yin, Yonghong Shao, Junle Qu, Haoming Lin, Hanben Niu

Proceedings Volume 6851, 68510N (2008) https://doi.org/10.1117/12.764304

KEYWORDS: Luminescence, Endoscopy, Speckle pattern, Speckle, Image processing, Diffusers, Point spread functions, Endoscopes, Confocal microscopy, Microscopy

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Proceedings Article | 8 January 2008 Paper

Wide-field fluorescence sectioning microscopy using dynamic speckle illumination

Jun Yin, Junle Qu, Yonghong Shao, Lingling Zhao, Haoming Lin, Hanben Niu

Proceedings Volume 6826, 682615 (2008) https://doi.org/10.1117/12.757042

KEYWORDS: Luminescence, Microscopy, Diffusers, Speckle, Speckle pattern, Confocal microscopy, Point spread functions, Glasses, Image processing, Microscopes

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Proceedings Article | 8 January 2008 Paper

A novel field of view zoom scanning protocol for simultaneous time- and spectrum-resolved multifocal multiphoton microscopy

Junle Qu, Lixin Liu, Ziyang Lin, Lei Wang, Wenqing Liu, Hanben Niu

Proceedings Volume 6826, 682636 (2008) https://doi.org/10.1117/12.760252

KEYWORDS: Luminescence, Zoom lenses, Image resolution, Mirrors, Multiphoton microscopy, Imaging systems, Microlens array, Optical scanning systems, Optical components, Spectral resolution

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Proceedings Article | 19 July 2007 Paper

In vivo measurement of the carotenoid level using portable resonance Raman spectroscopy

Yonghong Shao, Junle Qu, Yonghong He

Proceedings Volume 6628, 662817 (2007) https://doi.org/10.1117/12.726859

KEYWORDS: Raman spectroscopy, Raman scattering, Tissue optics, Signal to noise ratio, In vivo imaging, Light scattering, Luminescence, Argon ion lasers, Tissues, Scattering

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Proceedings Article | 27 October 2006 Paper

Contrast enhancement subsurface optical imaging with different incident polarization states

Hanrong Shao, Yonghong He, Yonghong Shao, Hui Ma

Proceedings Volume 6047, 60470Z (2006) https://doi.org/10.1117/12.710696

KEYWORDS: Polarization, Photons, Scattering, Light scattering, Metals, Optical imaging, Tissues, Biomedical optics, Lawrencium, Geometrical optics

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Proceedings Article | 27 October 2006 Paper

A wide linear range surface plasmon resonance biosensor

Zhanliang Sun, Yonghong He, Yonghong Shao, Jihua Guo

Proceedings Volume 6047, 604734 (2006) https://doi.org/10.1117/12.710697

KEYWORDS: Sensors, Surface plasmons, Gold, Modulation, Phase measurement, Interferometry, Biosensors, Polarization, Refractive index, Biological research

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Proceedings Article | 23 August 2002 Paper

Novel interleaver based on modified Michelson interferometer with three-mirror Fabry-Perot interferometer and Gires-Tournois resonator

Yonghong Shao, Yaoliang Jiang, Quan Zheng, Longsheng Qian

Proceedings Volume 4906, (2002) https://doi.org/10.1117/12.480622

KEYWORDS: Resonators, Michelson interferometers, Fabry–Perot interferometry, Fabry–Perot interferometers, Reflectivity, Mirrors, Wavelength division multiplexing, Beam splitters, Fiber optic communications, Optical filters

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Proceedings Article | 23 August 2002 Paper

Design of Gires-Tournois resonator dispersion compensator

Yonghong Shao, Yaoliang Jiang, Quan Zheng, Longsheng Qian

Proceedings Volume 4906, (2002) https://doi.org/10.1117/12.480548

KEYWORDS: Resonators, Mirrors, Interferometers, Reflectivity, Fabry–Perot interferometry, Optical communications, Mechanics, Physics, Phase interferometry, Dispersion

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Showing 5 of 22 publications

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