Proceedings Article | 3 March 2022
KEYWORDS: Skin, 3D image processing, Microscopy, 3D modeling, Second-harmonic generation, Luminescence, Multiphoton microscopy, Collagen, Zinc oxide, Signal detection
Multiphoton microscopy (MPM) has been discovered for several decades now. With noninvasive nature, subcellular resolution, relatively high imaging depth, distinctive biochemical-based imaging contrast, and the ability to be combined with other imaging and spectroscopic modes, MPM has been applied in many different fields in characterizing biological tissues and organisms, including medical diagnosis and treatment, drug development and penetration, cosmetic research, etc. In particular, its application in cosmetic industry has been advancing exponentially. This review will be focusing on a brief historical review and some recent advancements of MPM with a focus on its applications in cosmetic research. With the developments in both the hardware and software, such as the recent improvements in tunable laser sources, scanning speed and angles, the speed of computing, real-time image processing, etc., MPM will continue to grow dramatically. Together with other imaging and spectroscopic modalities, it is envisioned that higher imaging resolution, larger image field, faster image acquisition and processing, as well as more complementary information will be achieved, and will help revolutionize the field of cosmetic research.
