The waveguide structure for the local evanescent array coupled (LEAC) biosensor is optimized theoretically
with Beam Propagation Method (BPM) simulations. The LEAC biosensor has successfully demonstrated
experimental results of a sensitivity of 16% /nm and a metrology limit of 14 pm. Considering the waveguide
thickness detector position used in previous experiments are far from optimized values, the detection performance of
the LEAC sensor can be significantly improved with the simulated optimal structure. With the optimized
parameters, when the upper cladding is air the estimated metrology limit is 0.8 pm; with water as the upper cladding
for real-time measurements in an intigrated microfluidic channel, the estimated metrology limit is 1.6 pm.
A multi-channel fiber optic photoluminescence system was developed for real time, in situ and continuous
quantitative monitoring of chemical parameters in analytes, with the advantages of ease of use and low cost in
manufacturing. The system sequentially excites 8 optodes and measures the resulting photoluminescence with a single
photomultiplier. The oxygen sensitive phosphorescent dye or pH sensitive fluorescent dye in the optodes transduces the
activity of an enzyme genetically engineered to detect a specific chemical species such as a chlorinated ethene. System
operation is described and the relative signal is compared to measurements made with single channel system based on a
commercial spectrometer.
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