Dr. Zhe Wang Profile

Dr. Zhe Wang

at University of Naples "Federico II"

SPIE Involvement:

Author

Proceedings Article | 11 August 2023 Presentation + Paper

Multimodal characterization of thin polymeric film by digital holography-based methods

Zhe Wang, Vincenzo Ferraro, Lisa Miccio, Veronica Vespini, Vittorio Bianco, Francesca Ferranti, Silvia Mari, Pietro Ferraro, Pier Luca Maffettone, Sara Coppola

Proceedings Volume 12622, 126220R (2023) https://doi.org/10.1117/12.2675337

KEYWORDS: Digital holography, Holography, Film thickness, Thin films, Polymer thin films, Holograms, Polymers, Nanoparticles

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Proceedings Article | 11 August 2023 Poster + Paper

Customized iris diaphragm enables quantitative evolution from liquid droplet to free-standing thin film

Vincenzo Ferraro, Zhe Wang, Lisa Miccio, Veronica Vespini, Volodymyr Tkachenko, Sara Coppola, Pier Luca Maffettone

Proceedings Volume 12622, 126221A (2023) https://doi.org/10.1117/12.2675330

KEYWORDS: Thin films, Film thickness, Liquids, Iris, Holography, Digital holography, Fabrication, Viscosity, Holographic interferometry

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Proceedings Article | 11 August 2023 Presentation + Paper

Three-dimensional holographic particle tracking in space-time digital holography

Zhengzhong Huang, Zhe Wang, Daniele Pirone, Vittorio Bianco, Pasquale Memmolo, Liangcai Cao, Pietro Ferraro

Proceedings Volume 12622, 126220T (2023) https://doi.org/10.1117/12.2675460

KEYWORDS: Microfluidics, Holography, Holograms, 3D tracking, Digital holography, Particles, Interferograms, Phase shifting, Flow cytometry, Complex amplitude

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Proceedings Article | 11 August 2023 Poster + Paper

Phase-contrast tomography of plant cells’ nuclei enabled by reversible induced dehydration

Zhe Wang, Daniele Pirone, Vittorio Bianco, Pasquale Memmolo, Massimiliano Villone, Pier Luca Maffettone, Pietro Ferraro

Proceedings Volume 12622, 1262219 (2023) https://doi.org/10.1117/12.2675076

KEYWORDS: Tomography, Digital holography, Biological imaging, Phase contrast, Holography, Holograms, Refractive index, Beam splitters, Microscopy

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Proceedings Article | 11 August 2023 Presentation + Paper

Phase-retrieval in scanning digital holography for optofluidics

Zhe Wang, Vittorio Bianco, Pier Luca Maffettone, Pietro Ferraro

Proceedings Volume 12622, 126220V (2023) https://doi.org/10.1117/12.2675075

KEYWORDS: Digital holography, Microfluidics, Holograms, Optofluidics, Sensors, Holography, Cameras, Calibration, Reconstruction algorithms, Microscopy

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Proceedings Article | 11 August 2023 Poster + Paper

Motility-based screening of probiotic bacteria candidates by label-free coherent light tester

Vittorio Bianco, Filomena Nazzaro, Zhe Wang, Jaromir Behal, Simonetta Grilli, Pietro Ferraro

Proceedings Volume 12622, 1262216 (2023) https://doi.org/10.1117/12.2674359

KEYWORDS: Bacteria, 3D tracking, Biospeckle, Intestine, Microscopy, Digital holography, Control systems, Particles, Microscopes, Intelligence systems

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SPIE Journal Paper | 19 January 2023

Multimodal quantitative phase contrast characterization of thin polymeric film

Zhe Wang, Fabiana Graziano, Ciro Tortora, Federico Olivieri, Rachele Castaldo, Marino Lavorgna, Gennaro Gentile, Lisa Miccio, Veronica Vespini, Vittorio Bianco, Pietro Ferraro, Sara Coppola, Pier Luca Maffettone

OE, Vol. 62, Issue 04, 041405, (January 2023) https://doi.org/10.1117/12.10.1117/1.OE.62.4.041405

KEYWORDS: Polymers, Polymer thin films, Particles, Holography, Film thickness, Thin films, Scanning electron microscopy, Holograms, Phase contrast, Digital holography

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Proceedings Article | 31 May 2022 Presentation + Paper

New needs to measure soft matter at micro-/nanoscale: a new possibility thanks to digital holography

Zhe Wang, Lisa Miccio, Sara Coppola, Vittorio Bianco, Pasquale Memmolo, Volodymir Tkachenko, Vincenzo Ferraro, Ernesto Di Maio, Pier Luca Maffettone, Pietro Ferraro

Proceedings Volume 12137, 121370G (2022) https://doi.org/10.1117/12.2625116

KEYWORDS: Holography, Particles, Digital holography, Polymers, Thin films, Liquids, 3D metrology, Printing, Manufacturing, Interferometry

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Proceedings Article | 20 May 2022 Paper

Tomographic phase microscopy at single cell scale without a-priori knowledge of cell orientations: smart strategies for rotation angles recovery

Lisa Miccio, Daniele Pirone, Daniele Sirico, Francesco Merola, Pasquale Memmolo, Vittorio Bianco, Zhe Wang, Jaromir Behal, Danila Del Giudice, Martina Mugnano, Pietro Ferraro

Proceedings Volume 12136, 121360T (2022) https://doi.org/10.1117/12.2626452

KEYWORDS: Tomography, Microscopy, Microfluidics, Digital holography, Holography, 3D image processing, Cameras, Artificial intelligence, Refractive index, Optical design

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Tomographic phase microscopy in cytometry environment is feasible at single cell level and without the a-priori knowledge of the cell orientation. In the present paper we demonstrate different strategies for recovery the rotation angles of single cells and clusters when rotating into microfluidic channels, thus realistically opening to the implementation of marker-free cytofluorimeter for three-dimensional imaging of biological fluids. The pioneering developed strategies allows to measure quantitatively the inner distribution of the refractive indexes inside the cell volume avoiding the use of chemical and fluorescent tags. The imaging apparatus is based on label-free Digital Holography in microscopy setup designed in transmission geometry to image 700x700μm Field of View with lateral resolution of 0.5μm. Digital Holography is perfectly suited for imaging in microchannels as it allows the numerical refocusing of sample into a three-dimensional volume. In the present paper, such imaging arrangement is combined with a high-precision pumping system connected to a microfluidic channel that allow the complete rotation of the flowing cells into the Field of View. High-speed 25Megapixel camera acquires holographic set measurements of all rotating cells that are numerically processed to obtain quantitative two-dimensional phase-contrast maps at different view angles. Accurate numerical algorithms allow to tag each phasecontrast maps with the rotation angle in the microchannel. The couples made of phase-contrast map and measured angle are given as input at tomography algorithms to obtain the refractive index distribution into the cell volume. The approach in principles works properly for any kind of biological matter subjected to rotation as already demonstrated in case of nuclei of plant cells during dehydration. Furthermore, the same approach allows to show the three-dimensional distribution of internalized nano-particles as in case of nano-graphene oxide. The most important achievement and innovation of such strategy is the high-throughput phase-contrast tomography at single cell level that opens to new diagnostic tool thanks to the possibility to have statistically relevant measurement on cell population and also for the possibility to use artificial intelligence architecture for cell identification and classification.

Proceedings Article | 20 June 2021 Presentation + Paper

Investigation of plant cells intracellular dynamics by digital holography

Zhe Wang, Vittorio Bianco, Daniele Pirone, Pasquale Memmolo, Massimiliano Maria Villone, Pier Luca Maffettone, Pietro Ferraro

Proceedings Volume 11786, 117860M (2021) https://doi.org/10.1117/12.2592678

KEYWORDS: Digital holography, Phase contrast, Holography, Refractive index, Phase imaging, Water, Visualization, Spherical lenses, Planets, Pharmacy

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Proceedings Article | 20 June 2021 Presentation + Paper

Quantitative thickness mapping of a freestanding thin liquid film by fusing digital holography and white light interferometry

Vincenzo Ferraro, Zhe Wang, Lisa Miccio, Pier Luca Maffettone

Proceedings Volume 11786, 117860L (2021) https://doi.org/10.1117/12.2592662

KEYWORDS: Thin films, Liquids, Digital holography, Optical interferometry, Holography, Interferometry, Quantitative analysis, Image registration, Chemistry, Calibration

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Showing 5 of 11 publications

Conference Committee Involvement (1)

Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VII

23 June 2025 | Munich, Germany

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