Early studies suggest that some PDT photosensitizers can be used as sonosensitizer for sonodynamic therapy (SDT). In this study, sonodynamic effects of a novel water soluble chlorin YLG-1 were evaluated. SDT of YLG-1 solution was carried out using an ultrasound transducer of 450 KHz at 2.0 W/cm2 for 0 – 600 s. Comparison of spectroscopic profiles of YLG-1 before and after SDT demonstrated that sonification could cause the reduction of YLG-1 absorbance and fluorescence emission but did not cause obvious change of the Raman spectrum. No significant by-products were identified from spectroscopic study. Nevertheless, this study demonstrated that the sonification mediated sensitizer fluorescence bleaching effect could be partially blocked by the addition of NaN3, which suggests a possible involvement of singlet oxygen in the process of sonification of YLG-1 compound.
KEYWORDS: Photodynamic therapy, Digital photography, Skin, Photography, RGB color model, Quantitative analysis, Software development, Color difference, Light sources and illumination
Portwine stain (PWS) birthmarks are congenital vascular malformations of the skin. Pulse dye laser (PDL) is current treatment option for PWS. As a possible alternative, vascular targeting photodynamic therapy (PDT) is currently under formal clinical trials for the treatment of PWS in China. Color blanching is an important indicator of treatment effectiveness. There is a need to develop an objective and quantitative color analysis system for the evaluation of PWS color before and after PDT treatment.In this study, the CIE L*a*b* color space coordinate was used to quantify skin and PWS coloron digital color photos.A custom-made multi-color template (PWS Color Card) for the accurate color correction of digital photos. Hardware and software were developed and tested for quantitative PWS color and size analyses before and after PDT treatment. Results suggest that PWS color, its color blanching and treatment efficacy in PDT treatment can be quantitatively analyzed using digital photos in conjunction with suitable color analysis and color correction algorithm.
Photodynamic therapy (PDT) utilizes the combined action of photosensitizer, light, and molecular oxygen to generate reactive singlet oxygen (1O2) to treat various diseases. Photosensitizer is a key component in PDT and its singlet oxygen quantum yield determines the effectiveness of its photodynamic reaction. This study evaluated the singlet oxygen production of a novel water soluble chlorin photosensitizer (YLG-I) using a 1O2-specificfluorescence probe - Singlet Oxygen Sensor Green reagent (SOSG). The spectra and singlet oxygen luminescence in different solvents were evaluated using mTHPC as a reference. It was estimated and verified that the singlet oxygen quantum yield of the new photosensitizer YLG-I was at the same level of the potent chlorin sensitizer mTHPC. Preliminary evaluation suggested that YLG-I was a promising photosensitizer for PDT.
Photodynamic therapy (PDT) utilizes the combined action of photosensitizer, light and molecular oxygen to generate singlet oxygen to treat various diseases. Photosensitizer is a key component and its optical properties and singlet oxygen quantum yield determine the effectiveness of its photodynamic reaction. This study evaluated the singlet oxygen production of a novel water solubility chlorin photosensitizer - namely YLG-I, which was developed in China. The absorption spectra and singlet oxygen luminescence different solvents were evaluated. It was estimated that the singlet oxygen quantum yield of the new photosensitizer YLG-I was at the same level of the potent chlorin sensitizer mTHPC. Preliminary evaluation suggested that YLG-I was a promising photosensitizer for PDT. This presentation will report the detection of singlet oxygen luminescence of the newly developed PDT sensitizer.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.