Stimulated emission depletion (STED) optical nanoscopy can achieve super-resolution fluorescence imaging, by suppressing fluorescence on the peripheral of excitation center with a 0-2pi spiral phase plate. Previously, the STED intensity distribution at the focal plane and the derived expression of resolution are generally analytical described by vectorial integral. To overcome the complex and multifarious of the vectorial calculation, we proposed scalar integral method and used the Collins-Huygens integral to analytically describe the peak intensity, the central intensity of the doughnut spot and the resolution of STED nanoscopy. We verified our method by comparing our results with vector theory. And we found it agreed well with vectorial theory under the high STED power, which was commonly used experimental condition for high resolution. Our method provides a fast and convenient way to evaluate the performance of STED with spiral phase modulation.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.